Why does MAFA (D2Z6N) Rabbit mAb #79737 recognize a band at approximately 48 kDa when the predicted molecular weight is approximately 37 kDa?

MAFA is a phosphoprotein. The multiple phosphorylation sites on this protein cause a mobility shift, resulting in the protein migrating slower by gel electrophoresis. This has been confirmed by treatment with CIAP, which has been shown to result in a shift in MAFA’s mobility from approximately 48 kDa to a more rapidly migrating form of approximately 40 kDa (see PMID: 17682063 Figure 1A; https://pubmed.ncbi.nlm.nih.gov/17682063/).



 

Last updated: February 28, 2024