Why does the observed molecular weight differ from the predicted molecular weight when using your COL1A1 antibodies?

When performing western blot using our COL1A1 antibodies, we detect the propeptide form of COL1A1, which is observed around 220kDa. The propeptide COL1A1 is processed to a mature form by cleavage of the N-terminal 1-161AA chain and the C-terminal 1219-1464AA portion resulting in a ~140kDa protein (UniProt ID P02452). This mature form may be detected by our COL1A1 antibodies when analyzing tissue samples but is not commonly observed in our hands in cell lysates.

Last updated: February 23, 2024