Why is there a doublet at 60 kDa for Akt?

It is not uncommon for Akt to appear as a doublet on a western blot. This double banding is due to differential phosphorylation of the different Akt isoforms. If a doublet is seen with a phospho-specific Akt antibody, it should also be seen with a total Akt antibody. From referencing our in-house testing, the doublet is more commonly seen with mouse samples.

Last updated: March 25, 2026