Will your Anti-biotin, HRP-linked Antibody #7075 cross-react with endogenous biotin in cell lysates?

Our Anti-biotin, HRP-linked Antibody #7075 is intended for use with our Biotinylated Protein Ladder Detection Pack #7727. However, it is possible for this antibody to also detect endogenously biotinylated proteins in cell lysates if present in sufficient quantities. If this type of interaction is suspected in a given experiment, it is recommended that the Biotinylated Protein Ladder be probed separately from the cell lysate lanes to avoid detection of endogenously biotinylated proteins in the experimental samples.

Last updated: April 16, 2024