Additional Reagents (Not Supplied):
NOTE: Suggested dilutions and volumes are provided for reference. Optimal conditions for SA-beta-Gal Fluorescent Substrate performance may vary by cell type and container type.
NOTE: Cell treatments to induce senescence should be completed before initiating staining with this kit. Ensure that an untreated control is included, to provide a baseline measurement of fluorescence for comparison.
NOTE: Formaldehyde fixation may be performed at the completion of the incubation step with the SA-beta-Gal Fluorescent Substrate #38154. Fixation with up to 4% formaldehyde for up to 15 minutes will not significantly reduce the fluorescence of the substrate. However, permeabilization with methanol or Triton X-100 will cause a significant reduction in fluorescence. If permeabilization is required for immunolabeling of intracellular targets, conduct an experiment to determine whether this will significantly compromise detection of the fluorescent substrate in that cell model.
posted April 2010
Protocol Id: 1986
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