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7720
Prestained Protein Marker, Broad Range (Premixed Format)
Experimental Controls
MW Marker

Prestained Protein Marker, Broad Range (Premixed Format) #7720

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# Product Name Application Reactivity
  • WB
Prestained Protein Marker, Broad Range (Premixed Format): Image 1

Prestained Protein Marker, Broad Range (Premixed Format): Image 2

Product Usage Information

(a) Transfer the desired amount of the Prestained Protein Marker to a separate tube. For visualizing during electrophoresis: use 15 µl for mini-gels and 30 µl for full length gels.

(b) Heat the Marker to 95-100ºC for 3-5 minutes.

(c) After a quick microcentrifuge spin, load directly onto a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells.

Note on Apparent Molecular Weights:

The relative sizes of these protein markers may depend on the type of gel used and may appear different than expected. The coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and will likely alter its mobility in an SDS polyacrylamide gel. The extent of this effect can vary with the properties of the gel type (e.g., Tris-glycine, Tris-Tricine, etc) used in the analysis. For this reason, the sizes of these marker proteins are expressed here as apparent molecular weights. For best results, we recommend using these prestained protein markers on a Tris-glycine SDS gel.

Solutions and Reagents

Contains 0.1–0.2 mg/ml of each protein

Storage

Store at -20°C.

Prestained Protein Marker, Broad Range

Protein Source Apparent MW (Da)
MBP-β-galactosidase E. coli 175,000
MBP-paramyosin E. coli 80,000
MBP-CBD E. coli 58,000
CBD-Mxe Intein-2CBD E. coli 46,000
CBD-Mxe Intein E. coli 30,000
CBD-BmFKBP13 E. coli 25,000
Lysozyme chicken egg white 17,000
Aprotinin bovine lung 7,000
CBD
chitin binding domain
MBP
maltose-binding protein.

Product Description

Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands between 7 and 175 kDa when electrophoresed (1). The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins. The apparent molecular weight of the prestained proteins are given in the table below.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
This product is produced at New England Biolabs.

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