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We recommend the following alternatives

W     IF F   H
W IP         H
REACTIVITY SENSITIVITY MW (kDa) SOURCE
70 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

AFP Antibody detects recombinant human and mouse AFP protein. The antibody can only immunoprecipitate human AFP.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human AFP. Antibodies are purified by protein A and peptide affinity chromatography.

Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).


1.  Meier, V. et al. (2006) Comp Hepatol 5, 2.

2.  Warnes, T.W. and Smith, A. (1987) Baillieres Clin Gastroenterol 1, 63-89.

3.  Peng, S.Y. et al. (2004) Int J Cancer 112, 44-50.


Entrez-Gene Id 174
Swiss-Prot Acc. P02771


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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AFP Antibody