View Featured Offers >>
9930
Apoptosis Antibody Sampler Kit (Mouse Preferred)
Primary Antibodies
Antibody Sampler Kit
  1. Products
  2. Primary Antibodies
  3. Apoptosis Antibody Sampler Kit (Mouse Preferred)

Apoptosis Antibody Sampler Kit (Mouse Preferred) #9930

Reviews ()
Citations (17)
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 1
Simple Western™ analysis of lysates (1 mg/mL) from Jurkat cells treated with Cytochrome C using Caspase-3 (D3R6Y) Rabbit mAb #14220. The virtual lane view (left) shows the target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 2
Simple Western™ analysis of lysates (0.1 mg/mL) from Jurkat cells treated with Cytochrome C using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664. The virtual lane view (left) shows the target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 3
Western blot analysis of various cell lines, untreated (-) or treated with Staurosporine #9953 (1 μM; 3 hr) or with Etoposide #2200 (25 μM, overnight), using Caspase-3 (D3R6Y) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). MCF7 cells are negative for caspase-3 expression.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 4
Western blot analysis of extracts from L-929 cells, untreated (-) or treated with Brefeldin A #9972 (10 μM, 30 hr; +), using Caspase-12 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 5
Western blot analysis of extracts from BaF3, Raw264.7 and C2C12 cell lines, using Caspase-8 Antibody (Mouse Specific).
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 6
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 7
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 8
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct overexpressing mouse caspase-8 (mCasp8) (+), using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific).
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 9
Western blot analysis of extracts from NIH/3T3 cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using Caspase-9 Antibody. p39: caspase-9 cleaved at Asp368. p37: caspase-9 cleaved at Asp353.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 10
Western blot analysis of extracts from L929 cells, untreated or staurosporine-treated, using Cleaved Caspase-9 (Asp353) Antibody (upper) or Caspase-9 Antibody #9504 (lower).
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 11
Western blot analysis of NIH/3T3 cells, untreated or staurosporine-treated (1 µM), using Cleaved PARP (Asp214) (7C9) Mouse mAb.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 12
Western blot analysis of extracts from C6 (rat), NIH/3T3 (mouse), and Jurkat (human) cells, untreated or treated with staurosporine #9953 (1uM, 3hrs) or etoposide #2200 (25uM, 5hrs) as indicated, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 13
Western blot analysis of extracts from HCT116 cells (lane 1) or CASP3 knock-out cells (lane 2) using Caspase-3 (D3R6Y) Rabbit mAb #14220 (upper), and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The absence of signal in the CASP3 knock-out HCT116 cells confirms specificity of the antibody for CASP3.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 14
Western blot analysis of extracts from CTLL-2 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by hTNF-α #8902 (20 ng/ml, 4 hours), using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific).
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 15
Immunoprecipitation of extracts from Jurkat cells, untreated or etoposide-treated (25uM, 5hrs), using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb. Western blot was performed using the same antibody.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 16
Confocal immunofluorescent analysis of Raw 264.7 cells, untreated (left) or treated with TNF-α and Cycloheximide (20 ng/ml and 2.5 ug/ml, 4 hours and 3 hours; right), using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 17
Confocal immunofluorescent analysis of NIH/3T3 cells, staurosporine-treated (left) or untreated (right), using Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 18
Flow cytometric analysis of A20 cells, untreated (blue) or treated with etoposide #2200 (25 uM, overnight; green) using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 19
Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb in the presence of control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide (#1050) (right).
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 20
Immunohistochemical analysis using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb on SignalSlide® Cleaved Caspase-3 IHC Controls #8104 (paraffin-embedded Jurkat cells, untreated (left) or etoposide-treated (right)).
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 21
Immunohistochemical staining of paraffin-embedded mouse embryo, showing cytoplasmic localization in apoptotic cells, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 22
Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine #9953 treated (right) labeled with Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin #8953 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Apoptosis Antibody Sampler Kit (Mouse Preferred): Image 23
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with etoposide #2200 (green), using Cleaved Caspase-3(Asp175) (5A1E) Rabbit mAb compared to a nonspecific negative control antibody (red).
Image Gallery
Learn more about how we get our images
To Purchase # 9930
Cat. # Size Qty. Price
9930T		 1 Kit  (8 x 20 microliters)
$ 645

Custom Formulation

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Caspase-3 (D3R6Y) Rabbit mAb 14220 20 µl
  • WB
  • IP
 H M R Mk 35, 19, 17 Rabbit IgG
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 9664 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
 H M R Mk 17, 19 Rabbit IgG
Caspase-8 Antibody (Mouse Specific) 4927 20 µl
  • WB
 M 45, 57 Rabbit 
Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) 8592 20 µl
  • WB
  • IP
  • IF
  • F
 M 18, 43 Rabbit IgG
Caspase-9 Antibody (Mouse Specific) 9504 20 µl
  • WB
 M 37, 39, 49 Rabbit 
Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) 9509 20 µl
  • WB
  • IF
 M 37 Rabbit 
Cleaved PARP (Asp214) (7C9) Mouse mAb 9548 20 µl
  • WB
 M 89 Mouse IgG2b
Caspase-12 Antibody 35965 20 µl
  • WB
 M 42, 55 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
 Rab Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
 M Horse 

Product Description

The Apoptosis Antibody Sampler Kit (Mouse Specific) is designed for use with mouse samples and offers an economical means to evaluate the levels of active and inactive caspases. The kit contains enough primary and secondary antibodies to perform two Western blot experiments with each antibody.

Specificity / Sensitivity

Each antibody in the Apoptosis Antibody Sampler Kit (Mouse Preferred) recognizes endogenous levels of its respective target. Caspase-3 (D3R6Y) Rabbit mAb detects full-length caspase-3 (35 kDa) as well as the large subunit (17/19 kDa) of caspase-3 resulting from cleavage during apoptosis. Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb detects the large fragment (17/19 kDa) of caspase-3 resulting from cleavage adjacent to Asp175. Caspase-8 Antibody (Mouse Specific) detects full length (57 kDa) and the cleaved intermediate (p43) of mouse caspase-8. Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) detects the p18 subunit of mouse caspase-8 resulting from cleavage at Asp387and the cleavage product containing the pro-domain (p43). Caspase-9 Antibody (Mouse Specific) detects both full length (49 kDa) and the large fragments of mouse caspase-9 resulting from cleavage at aspartic acid 353 (37 kDa) and/or aspartic acid 368 (39 kDa). Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) detects the 37kDa subunit of mouse caspase-9 only after cleavage at aspartic acid 353. Non-specific proteins that are induced by apoptosis under certain conditions may be detected. Caspase-12 Antibody detects full-length mouse caspase-12 protein (55 kDa) and its cleaved product (42 kDa). Cleaved PARP (Asp214) (7C9) mouse mAb detects the large fragment (89 kDa) of PARP1 resulting from caspase cleavage.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Asp387 of mouse caspase-8 protein, or carboxy-terminal residues surrounding Asp214 in mouse PARP protein. Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the p20 subunit of human caspase-3 protein. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to a region within the large 18 kDa subunit of mouse caspase-8 protein, residues adjacent to Asp353 of mouse caspase-9 protein, or residues surrounding amino acid 158 of mouse caspase-12 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 8, 9, 10 and 12) are closely coupled to proapoptotic signals. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, α-fodrin, DFF and lamin A, and induce apoptosis. Cytochrome c released from mitochondria is coupled to the activation of caspase-9, a key initiator caspase (1). Proapoptotic stimuli include the FasL, TNF-α, DNA damage and ER stress. Fas and TNFR activate caspases 8 and 10 (2), DNA damage leads to the activation of caspase-9 and ER stress leads to the calcium-mediated activation of caspase-12 (3). The inhibitor of apoptosis protein (IAP) family includes XIAP and survivin and functions by binding and inhibiting several caspases (4,5). Smac/Diablo, a mitochondrial protein, is released into the cytosol upon mitochondrial stress and competes with caspases for binding of IAPs. The interaction of Smac/Diablo with IAPs relieves the inhibitory effects of the IAPs on caspases (6).
  1. Baker, S.J. and Reddy, E.P. (1998) Oncogene 17, 3261-3270.
  2. Budihardjo, I. et al. (1999) Annu. Rev. Cell Dev. Biol. 15, 269-290.
  3. Nakagawa, T. et al. (2000) Nature 403, 98-103.
  4. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
  5. Li, F. et al. (1998) Nature 396, 580-584.
  6. Du, C. et al. (2000) Cell 102, 33-42.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.