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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Caspase-3 (8G10) Rabbit mAb 9665 20 µl
Western Blotting Immunoprecipitation
H M R Mk 17, 19, 35 Rabbit IgG
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 9664 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 17, 19 Rabbit IgG
Caspase-8 Antibody (Mouse Specific) 4927 20 µl
Western Blotting
M 45, 57 Rabbit 
Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) 8592 20 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
M 18, 43 Rabbit IgG
Caspase-12 Antibody 2202 20 µl
Western Blotting
M 42, 55 Rabbit 
Caspase-9 Antibody (Mouse Specific) 9504 20 µl
Western Blotting
M 37, 39, 49 Rabbit 
Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) 9509 20 µl
Western Blotting Immunofluorescence
M 37 Rabbit 
Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) 9548 20 µl
Western Blotting
M 89 Mouse IgG2b
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting

Product Description

The Apoptosis Antibody Sampler Kit (Mouse Specific) is designed for use with mouse samples and offers an economical means to evaluate the levels of active and inactive caspases. The kit contains enough primary and secondary antibodies to perform two Western blot experiments with each antibody.

Specificity / Sensitivity

Each antibody in the Apoptosis Antibody Sampler Kit (Mouse Preferred) recognizes endogenous levels of its respective target. Caspase-3 (8G10) Rabbit mAb detects full-length (35 kDa) and the large fragment (17/19 kDa) of caspase-3 resulting from cleavage at aspartic acid 175. Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb detects the large fragment (17/19 kDa) of caspase-3 resulting from cleavage adjacent to Asp175. Caspase-8 Antibody (Mouse Specific) detects full length (57 kDa) and the cleaved intermediate (p43) of mouse caspase-8. Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) detects the p18 subunit of mouse caspase-8 resulting from cleavage at Asp387and the cleavage product containing the pro-domain (p43). Caspase-9 Antibody (Mouse Specific) detects both full length (49 kDa) and the large fragments of mouse caspase-9 resulting from cleavage at aspartic acid 353 (37 kDa) and/or aspartic acid 368 (39 kDa). Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) detects the 37kDa subunit of mouse caspase-9 only after cleavage at aspartic acid 353. Caspase-12 Antibody detects full-length mouse caspase-12 protein (55 kDa) and its cleaved product (42 kDa). Cleaved PARP (Asp214) (7C9) mouse mAb (Mouse Specific) detects the large fragment (89 kDa) of mouse PARP1 resulting from caspase cleavage.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3 protein, residues surrounding Asp387 of mouse caspase-8 protein, or carboxy-terminal residues surrounding Asp214 in mouse PARP protein. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to a region within the large 18 kDa subunit of mouse caspase-8 protein, residues adjacent to Asp353 of mouse caspase-9 protein, or residues surrounding amino acid 158 of mouse caspase-12 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 8, 9, 10 and 12) are closely coupled to proapoptotic signals. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, α-fodrin, DFF and lamin A, and induce apoptosis. Cytochrome c released from mitochondria is coupled to the activation of caspase-9, a key initiator caspase (1). Proapoptotic stimuli include the FasL, TNF-α, DNA damage and ER stress. Fas and TNFR activate caspases 8 and 10 (2), DNA damage leads to the activation of caspase-9 and ER stress leads to the calcium-mediated activation of caspase-12 (3). The inhibitor of apoptosis protein (IAP) family includes XIAP and survivin and functions by binding and inhibiting several caspases (4,5). Smac/Diablo, a mitochondrial protein, is released into the cytosol upon mitochondrial stress and competes with caspases for binding of IAPs. The interaction of Smac/Diablo with IAPs relieves the inhibitory effects of the IAPs on caspases (6).

1.  Baker, S.J. and Reddy, E.P. (1998) Oncogene 17, 3261-3270.

2.  Budihardjo, I. et al. (1999) Annu. Rev. Cell Dev. Biol. 15, 269-290.

3.  Nakagawa, T. et al. (2000) Nature 403, 98-103.

4.  Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.

5.  Li, F. et al. (1998) Nature 396, 580-584.

6.  Du, C. et al. (2000) Cell 102, 33-42.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

Apoptosis Antibody Sampler Kit (Mouse Preferred)