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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Basigin/EMMPRIN (E1S1V) Rabbit mAb #13287

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  • WB
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 38-58
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #95203.

    Protocol

    Specificity / Sensitivity

    Basigin/EMMPRIN (E1S1V) Rabbit mAb recognizes endogenous levels of total EMMPRIN protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala254 of human EMMPRIN protein.

    Background

    Basigin (EMMPRIN, CD147) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily (1). Basigin is a glycosylated protein with four known isoforms, of which isoform 2 is the most abundantly expressed (2). Multiple functions have been ascribed to Basigin; foremost among these is stimulating the secretion of extracellular matrix metalloproteinases by adjacent fibroblasts, a function which has been implicated in promoting tumor progression (2-4). Research studies have shown that Basigin is overexpressed by many tumor cells, and its expression level may correlate with tumor malignancy (5,6). A recent study identified the BASIGIN gene as a regulatory target of Slug, suggesting a role for Basigin in the process of epithelial-mesenchymal transition (7). Basigin has also been identified as a marker for a subset of highly suppressive regulatory T cells (8), and as an obligate receptor for the malarial parasite Plasmodium falciparum on human erythrocytes (9).

    Alternate Names

    Leukocyte activation; Matrix metalloproteinase inducer; OK blood group antigen

    For Research Use Only. Not for Use in Diagnostic Procedures.
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