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Render Timestamp: 2025-03-21T11:09:56.560Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:09:45.203
Product last modified at: 2025-01-01T09:04:11.964Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. CHD5 (D2F9Q) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CHD5 (D2F9Q) Rabbit mAb #44829

Filter:
  • WB
  • IP
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 220
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Immunohistochemistry (Paraffin) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CHD5 (D2F9Q) Rabbti mAb recognizes endogenous levels of total CHD5 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1564 of human CHD5 protein.

    Background

    CHD5 (chromodomain-helicase-DNA-binding-5) is one of the 9 known CHD proteins and is homologous to its family members, CHD3 and CHD4 (1-3). CHD5 interacts with the nucleosome remodeling and deacetylation (NuRD) complex (1,4). CHD5 is characterized by two PHD domains, two chromo domains, a SNF2-like helicase/ATPase domain, as well as a conserved coiled-coil motif in the C-terminal region (1). CHD5 binds to the N-terminus of Histone H3 via its PHD domains (1). CHD5 was first characterized as a tumor suppressor gene found to be frequently lost in neuroblastomas (1). Since its initial discovery, CHD5 has been studied extensively and has been implicated in numerous other cancers including gliomas, breast, lung, ovarian, and prostate cancers as well as in laryngeal squamous cell carcinomas and gallbladder carcinoma (1). CHD5 is thought to be a prognostic marker in neuroblastoma patients. High CHD5 levels are strongly correlated with favorable clinical outcomes, whereas low or absent expression is associated with MYCN amplification and poor outcomes (1). In addition, CHD5 has a dual function in neurogenesis, playing a transcription activating role in neurogenesis, while interacting with the Polycomb group proteins to repress genes encoding regulators of other lineages. Deletion of CHD5 also inhibits neuronal differentiation leading to the accumulation of undifferentiated progenitors (5).

    Database Links

    UniProt ID: Q8TDI0


    Entrez-Gene Id: 26038


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