Immunohistochemical analysis of paraffin-embedded human Crohn's disease of the colon using CXCR4 (D4Z7W) Rabbit mAb (IHC Specific).
Immunohistochemical analysis of paraffin-embedded Jurkat (left) and 293T (right) cell pellets using CXCR4 (D4Z7W) Rabbit mAb (IHC Specific).
Immunohistochemical analysis of paraffin-embedded human tonsil using CXCR4 (D4Z7W) Rabbit mAb (IHC Specific).
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
posted February 2010
revised March 2016
Protocol Id: 283
CXCR4 (D4Z7W) Rabbit mAb (IHC Specific) recognizes endogenous levels of total CXCR4 protein.Species Reactivity:
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domains of human CXCR4 protein.
CXCR4 is a chemokine receptor that belongs to the G protein-coupled receptor family. It is activated by a small cytokine, CXCL12, also known as stromal cell derived factor 1 (SDF-1) (1). The main function of CXCR4 is the mediation of the homing of progenitor cells in the bone marrow and their recruitment to sites of injury (2). More recently, CXCR4 has been studied, as a potential therapeutic target, in the context of autoimmune diseases (3) as well as cancer, as the receptor is involved in the regulation of migration, proliferation, and survival of cancer cells (4).
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