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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb 7649 20 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H M R 84, 91 Rabbit IgG
Phospho-Stat2 (Tyr690) Antibody 4441 20 µl
Western Blotting
H 113 Rabbit 
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb 9145 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H M R Mk 79, 86 Rabbit IgG
Phospho-Stat3 (Ser727) Antibody 9134 20 µl
Western Blotting Immunoprecipitation Chromatin Immunoprecipitation
H M R 86 Rabbit 
Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb 4322 20 µl
Western Blotting Immunofluorescence Flow Cytometry
H M 90 Rabbit IgG
Phospho-Stat6 (Tyr641) Antibody 9361 20 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H 110 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The Phospho-Stat Pathway Sampler Kit provides an economical means to evaluate the activation status of Stat molecules, including the phosphorylation of Stat1 at Tyr701, Stat2 at Tyr690, Stat3 at Tyr705/Ser727, Stat5 at Tyr694 and Stat6 at Tyr641. The kit includes enough primary and secondary antibody to perform two Western blot experiments.

Specificity / Sensitivity

Each phospho-Stat antibody in the kit recognizes only the phosphorylated form of its specific target.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptides corresponding to residues surrounding Tyr690 of human Stat2, Ser727 of mouse Stat3, or Tyr641 of human Stat6. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr701 of human Stat1 protein, Tyr705 of mouse Stat3, or residues surrounding Tyr694 of human Stat5a protein.

Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).

1.  Darnell, J.E. et al. (1994) Science 264, 1415-1421.

2.  Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.

3.  Caldenhoven, E. et al. (1996) J Biol Chem 271, 13221-7.

4.  Wen, Z. et al. (1995) Cell 82, 241-50.

5.  Yokogami, K. et al. (2000) Curr Biol 10, 47-50.

6.  Lim, C.P. and Cao, X. (1999) J. Biol. Chem. 274, 31055-31061.

7.  Bromberg, J.F. et al. (1999) Cell 98, 295-303.

8.  Su, L. et al. (1999) J. Biol. Chem. 274, 31770-31774.

9.  Dentelli, P. et al. (1999) J Immunol 163, 2151-9.

10.  Cattaneo, E. et al. (1999) Trends Neurosci. 22, 365-369.

11.  Frank, D.A. (1999) Mol Med 5, 432-56.

Entrez-Gene Id 6772 , 6773 , 6774 , 6776 , 6777 , 6778
Swiss-Prot Acc. P42224 , P52630 , P40763 , P42229 , P51692 , P42226

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

Phospho-Stat Antibody Sampler Kit