|9914T||1 Kit (6 x 20 microliters)||
|Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb 7649||20 µl||
||H M R||84, 91||Rabbit IgG|
|Phospho-Stat2 (Tyr690) Antibody 4441||20 µl||
|Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb 9145||20 µl||
||H M R Mk||79, 86||Rabbit IgG|
|Phospho-Stat3 (Ser727) Antibody 9134||20 µl||
||H M R||86||Rabbit|
|Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb 4322||20 µl||
||H M||90||Rabbit IgG|
|Phospho-Stat6 (Tyr641) Antibody 9361||20 µl||
|Anti-rabbit IgG, HRP-linked Antibody 7074||100 µl||
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptides corresponding to residues surrounding Tyr690 of human Stat2, Ser727 of mouse Stat3, or Tyr641 of human Stat6. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr701 of human Stat1 protein, Tyr705 of mouse Stat3, or residues surrounding Tyr694 of human Stat5a protein.
Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).
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