Immunohistochemical analysis of paraffin-embedded RH30 cell pellet (left, high-expressing) or MDA-MB-231 cell pellet (right, low-expressing) using SHMT2 (E8O8J) Rabbit mAb (IHC Specific).
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SHMT2 (E8O8J) Rabbit mAb (IHC Specific).
Immunohistochemical analysis of paraffin-embedded human adenoid cystic carcinoma of the trachea using SHMT2 (E8O8J) Rabbit mAb (IHC Specific).
Immunohistochemical analysis of paraffin-embedded human tonsil using SHMT2 (E8O8J) Rabbit mAb (IHC Specific) (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using SHMT2 (E8O8J) Rabbit mAb (IHC Specific).
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using SHMT2 (E8O8J) Rabbit mAb (IHC Specific).
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using SHMT2 (E8O8J) Rabbit mAb (IHC Specific) (left) or SHMT2 (E7F4Q) Rabbit mAb #33443 (right). These two antibodies detect independent, unique epitopes on human SHMT2. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemical analysis of paraffin-embedded human appendix using SHMT2 (E8O8J) Rabbit mAb (IHC Specific).
|Immunohistochemistry (Paraffin)||1:100 - 1:400|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114||SignalStain® Boost IHC Detection Reagent (AP, Rabbit) #18653|
|SignalStain® DAB Substrate Kit #8059||SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713|
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632|
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised June 2020
Protocol Id: 283
SHMT2 (E8O8J) Rabbit mAb (IHC Specific) recognizes endogenous levels of total SHMT2 protein. This antibody does not cross-react with SHMT1 protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val489 of human SHMT2 protein.
Serine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxymethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5, 10-methylene-THF (1, 2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4).
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