|8885||Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate)||
The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most flow cytometry applications.
Supplied in 10 mM HEPES, 150 mM NaCl, pH 7.4, 0.08% sodium azide. Store at 4°C. Protect from light for up to one year. Do not freeze.
Affinity purified goat anti-rabbit IgG (H+L) antibody is conjugated to R-phycoerythrin. This product has been optimized for use as a secondary antibody in flow applications. Excitation is 450-580 nm and peak emission is 590 nm.
Anti-Rabbit IgG (H+L), (PE Conjugate) reacts with heavy and light chain of most rabbit immunoglobulins. No cross-reactivity to other serum proteins has been detected.
Less than 1% cross-reactivity is observed with immunoglobulins from other species.
This product has been optimized for use as a secondary antibody in flow cytometry applications. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
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