Basket Updated
0
Items added
Apoptosis is a type of programmed cell death that occurs normally throughout the lifespan and can also occur in response to harmful stimuli. Apoptotic cells are identified by their altered morphology, caspase activation, and the presence of damaged DNA.
Cell viability is a measure of the proportion of live, healthy cells within a population. Assays to assess cell viability measure metabolic activity, ATP content, cell proliferation, or membrane integrity.
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Our validation of the Annexin V-FITC Early Apoptosis Detection Kit #6592 was performed using live cells in suspension, but the steps can be carried out on live adherent cells as well. If the option is available to scrape the cells from the growth surface and perform the staining in suspension, this would be the easiest way to follow the directions for use on the datasheet. Otherwise we suggest following the directions as closely as possible for adherent cells.
Cell states can be monitored using markers correlating to your process of interest. These assays and antibodies can be used to detect cell viability, senescence, proliferation, apoptosis, cytotoxicity and oxidative state.
Products and Related Resources for Cell Death and Viability SARS-CoV-2 Research
Cytotoxicity is the ability of an exogenous agent to damage or kill cells. Measuring cytotoxicity can help determine the effect of drugs on cell viability.
Apoptosis is a highly regulated form of programmed cell death that occurs in multicellular organisms throughout life and in response to cellular stress.
Apoptosis is a regulated form of programmed cell death (PCD) that occurs without external influence.
Learn more about how intracellular flow cytometry can be used to measure unique signaling events to do with apoptosis, pluripotency status and epigenetic activity.
Regulation of cell death due to viral infection is important for host survival. In this series, we look at pathways regulated through cellular responses to viruses.
Flow Cytometry (F) Protocol for the Detection of Transcription Factors with recommended companion products.
Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC): easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry, Methanol Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.
Listing of IHC-related scientific posters and papers involving scientists from Cell Signaling Technology.
An orthogonal strategy for antibody validation involves cross-referencing antibody-based results with data obtained using non-antibody-based methods.
Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) : easy to follow directions describing the step by step experimental procedure.
Learn about antibody diversity, host species, isotypes, and how antibodies are developed for research, clinical diagnostics, and therapeutic uses.
A comprehensive list of peer-reviewed publications from Cell Signaling Technology.
A troubleshooting guide for CUT&Tag–a ChIP-seq alternative with lower sample requirements, lower sequencing cost, and improved signal-to-noise for profiling chromatin and protein-DNA interactions.
Learn DNA library preparation for ChIP-seq and CUT&RUN using the Illumina® protocol, from end repair & adaptor ligation to PCR enrichment & cleanup.
It is critical that our Phospho-p53 (Ser15) Antibody #9284 be diluted into 5% w/v non-fat dry milk, 1X TBS, 0.1% Tween® 20 and incubated at 4°C with gentle shaking, overnight. Diluting the antibody in BSA will result in high background on the blot.
Western Blotting Protocol (Fluorescent): easy to follow directions describing the step by step experimental procedure.
The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique to probe protein-DNA interactions within the natural chromatin context of the cell.
This is a four-part series of tips to improve your ChIP protocol.
This is a four-part series featuring tips to improve your ChIP protocol.
CST offers flow validated antibodies for single cell mass cytometry (MC) and IHC validated antibodies for imaging mass cytometry (IMC)