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Discover the power of flow cytometry in cellular analysis and biomarker detection. Click here to learn more
Flow Cytometry is readily amenable to intracellular target detection and can be successfully applied to the study of complex signaling pathways
Flow cytometry enables you to save time and analyze many characteristics of your cells in one experiment, using classic principles of antibody detection.
Watch this 3-D animation to see how flow cytometry enables the simultaneous characterization of intracellular signaling states and phenotyping of rare cell populations in a heterogeneous sample.
While flow cytometry is usually used to identify cell types using phenotypic markers on the cell surface, it can also measure intracellular signaling.
Intracellular flow cytometry enables characterization of signaling networks at a single cell level in phenotypically distinct cell populations.
To perform intracellular flow cytometry, fixation and permeabilization of cells is required to allow for antibody penetration and subsequent binding to targets without disrupting cellular morphology.
Learn more about how intracellular flow cytometry can be used to measure unique signaling events to do with apoptosis, pluripotency status and epigenetic activity.
If you’re only looking at surface markers in your flow cytometry, you’re missing out! We’ll discuss several protocol approaches to multiplexing so that you can analyze both surface markers and intracellular targets.
How can intracellular and extracellular protein readouts be combined in flow cytometry? This video offers protocol advice from our flow expert.
Designing or troubleshooting flow cytometry experiments to analyze intracellular signaling? Here’s what you need to know.
Use these quick reference tables to determine compatible fluorophore combinations for flow cytometry experiments using parallel or co-linear excitation.
CST offers flow validated antibodies for single cell mass cytometry (MC) and IHC validated antibodies for imaging mass cytometry (IMC)
Flow Cytometry Live Cell Protocol: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry, Methanol Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.
Download the Antibody Staining Guide for Flow Cytometry to help demystify the antibody staining process and guide you through assay set-up step-by-step.
Flow Cytometry, Triton X-100 Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.
This video describes four situations where it may be more helpful to use flow cytometry than western blot.
Watch this 20-minute webinar for tips and tools to optimize simultaneous incubation of different antibodies, plus insignts into maximing multiplexing efficiency while maintaining assay quality.
Troubleshooting tips for successfully performing Intracellular Flow Cytometry using CST recommended protocols and antibodies validated for Flow.
Flow Cytometry Protocol: easy to follow directions describing the step by step experimental procedure.
Validation for flow cytometry antibodies from CST Cell Signaling Technology
Protocol for Combined Staining of Intracellular Proteins and Cell Surface Markers in Blood: easy to follow directions describing the step by step experimental procedure.
This webinar provides practical advice to optimize flow cytometry protocols to reveal essential signaling insights needed to uncover the activity and function of rare immune cell subsets.
