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There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.
Concerns About Shellfish Growth Causes Local Nonprofits and Private Industry to Team Up to Test Rivers for CO2 Levels
The key to identifying neurons, microglia, oligodendrocytes, and astrocytes lies in using antibodies that target protein biomarkers specifically expressed and localized within these cells.
This guide includes some of the most common markers used to detect neuronal and glial cells, which include oligodendrocytes, astrocytes, and microglia.
Expert-reviewed interactive diagrams providing current overviews of neuronal and glial cell markers, as well as links to products from CST.
Overview of Adhesion and Extracellular Matrix signaling networks, antibodies and related reagents, interactive pathway diagrams, and technical resources.
Compare Cell Signaling Technology's Erk antibodies against one another to determine optimal applications
A multiple-antibody strategy is a powerful approach to antibody validation. The most common method to achieve this is to immunoprecipitate (IP) the target with one antibody and subsequently detect it by western blotting with another antibody against the
Hallmarks of Antibody Validation-Complementary Strategies that Employ Western Blot, Immunohistochemistry (IHC), Immunofluorescence (IF/ICC), Flow Cytometry, Histone Antibody, ChIP-qPCR, ChIP-seq.
For antigenic targets where expression of the protein is very low or unknown, the use of recombinant proteins or exogenous expression in a surrogate cell line may be necessary for antibody validation.
Validation using recombinant proteins or exogenous expression in a surrogate cell line can be used for targets with low levels of protein expression.
Discover the top epigenetic markers that you should look out for in prostate cancer research.
Use SimpleChIP kits to perform high throughput ChIP-Sequencing and assay genome wide changes in histone modifications and transcription factor binding.
When the Rictor (D16H9) Rabbit mAb (Sepharose Bead Conjugate) #5379 and the Rictor (D16H9) Rabbit mAb #9476 were tested by IP, #5379 worked well, showing good target enrichment in the IP reactions and no target signal with beads alone or rabbit IgG control IP reactions. When #9476 was tested by IP (dilution 1:50) using magnetic beads, target signal was seen in all IP reactions, including the above mentioned negative controls. Considering this background issue, we decided not to approve #9476 for IP. It is possible that #9476 may perform by IP under some conditions, however we cannot recommend or guarantee it considering the background we have seen.
Immunoprecipitation (IP) is a technique used to enrich a specific protein from a heterogeneous cell or tissue extract using a target-specific antibody. Co-immunoprecipitation (co-IP) is the pull-down of intact protein complexes.
A multiple antibody strategy is a powerful approach to antibody validation. This provides confidence that antibodies are binding the correct biomolecule.
Case Study 4: Exceptional Performance of XP Monoclonal Antibodies from Cell Signaling Technology.
Find out which epigenetic markers are important drivers in mixd lineage leukemia, or MLL.
Protein-DNA interactions mediate epigenetic processes. Researchers studying these interactions need a means of analyzing when & where they occur on the genome.
Epigenetic regulation, including aberrant DNA methylation and histone modifications, have been linked to Alzheimer’s disease, Huntington’s disease, Parkinson’s disease, multiple sclerosis, and amyotrophic lateral sclerosis.
Learn about host-based and dye-conjugated methods for IF multiplexing, as well as a sequential labeling strategy that works with indirect detection.
A collection of website featuring signal transduction research, disease and mutation databases, and online publication resources.
Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) : easy to follow directions describing the step by step experimental procedure.
Compare Cell Signaling Technology's Akt antibodies against one another to determine optimal applications
We have not conducted any fluorescent western blot multiplex experiments with p53. Unfortunately, many p53 antibodies have antigenic regions that overlap or have not been precisely mapped. For human samples, our Phospho-p53 (Ser15) Antibody #9284 or our Phospho-p53 (Ser15) (E9Y4U) Rabbit mAb #82530 in combination with p53 (DO-7) Mouse mAb #48818 may work, however, the binding epitope of #48818 has not been mapped. Alternatively, our Phospho-p53 (Ser15) (16G8) Mouse mAb #9286 could be tried in combination with our p53 (E9B5W) Rabbit mAb #30313. #30313 is the only total p53 antibody that we know has an epitope that resides outside of the N-terminus in the p53 tetramerization domain.
Multiplex IHC - Being able to visualize multiple targets simultaneously is important in research fields like immunology & oncology. Learn more here.
Discover the most important epigenetic markers that contribute to the progression of diffuse intrinsic pontine glioma (DIPG).
SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure
Discover the epigenetic drivers that help drive MDS / AML cancers
Discover the key epigenetic drivers of breast cancer and the best products to target them.