Basket Updated
0
Items added
A chronological listing of case studies and peer-reviewed publications utilizing PTMScan proteomic technology developed by Cell Signaling Technology.
A collection of website featuring signal transduction research, disease and mutation databases, and online publication resources.
Fill out the form to contact the Proteomics Analytical Services team.
Video tutorials illustrate the science behind cellular processes and signaling pathways.
We use the Octet® RED96.
Video Tutorial on PI3K / Akt Signaling created by CST on upstream stimuli and downstream effects on cellular events (autophagy, mTOR)
A binary approach is one of the best ways to evaluate antibody specificity.
The activity of genes and their regulatory elements is, in part, governed by their cell type-specific chromatin organization.
An introduction to human protein kinases: protein kinases are key regulators of cell function.
Video training tutorial on stem cells covering pluripotency, differentiation, and lineage markers by CST Cell Signaling Technology
Cell Signaling Technology (CST) Enters Agreement with Roche for CDx Development
This is a four-part series featuring tips to improve your ChIP protocol.
Discuss the mechanisms and control of antiproliferation signaling from cytokines such as transforming growth factor beta (TGF-β) and their role in cancer progression
For PTMScan experiments, the stage tip, or small-scale peptide desalting is a critical part of the experiment for a number of reasons. Detailed instructions for performing stage tip peptide desalting are included at the end of each PTMScan kit protocol.The stage tip step will remove salts and other contaminants prior to mass spectrometry that can distract the mass spectrometer from peptide identification. Importantly, this step will also serve to reduce the amount of antibody that may co-elute with the peptides and contaminate the analytical column or LC. This will improve the longevity of the analytical column and lead to more reproducible data.Stage tips can be made using an 18 gauge needle and C-18 disks, according to the method published by Rappsilber et al (Anal. Chem. 2003, 75, 3, 663–670, 2002). Alternatively, the tips can be purchased from a number of suppliers.The key to a successful desalting step is using a high-capacity stage tip, or one that is suitable for ~10ug of…
At CST, we recommend fluorochrome-conjugated F(ab')2 fragment secondaries as they reduce the non-specific background that is usually associated with their whole molecule counterparts. Whole molecule secondaries have the highest capacity for dye conjugation and, thus, are likely to yield the brightest signal. Their major downside is background, as many cell types express Fc receptors that these are likely to stick to without sufficient blocking.F(ab')2 have had their Fc regions removed through pepsin digestion, leaving two antigen binding domains.F(ab')2 antibodies have reduced capacity for dye conjugation which may result in a slightly weaker signal when compared to their whole molecule counterpart. Despite this, we find this format to provide an optimal balance between signal and noise.Monovalent F(ab) fragments are highly penetrant due to their small size, but also yield extremely low intensity. In our experience, these are best utilized as blocking reagents in their unconjugated …
Announce partnership with this local 1% for the Planet startup to help share and promote best practices for sustainability. Also, to encourage other companies to do the same by promoting recent sponsorship of NSTC as part of our ESR activities.
This Apoptosome video is a 3D animation illustrating key steps in Caspase-mediated formation and activation of the apoptosome, leading to cell death.
CST Scientist Richard Cho shares his perspective on the 2017 Sf meeting, including new research findings about non-neuronal cells in the brain.
The accuracy of western blot results relies heavily on the quality of the primary antibody used. CST™ antibodies are validated using multiple experiments.
Yes, our Protease/Phosphatase Inhibitor Cocktail (100X) #5872 can be used in mass spectrometry (MS) experiments. This cocktail does not contain AEBSF, which can cause peaks to shift.
CST product packaging is based on feedback from customers as to the number of assays they typically run.
The adaptive immune response uses antigen-specific receptors to detect foreign antigens with help from T cells, B cells, and NK T cells.
Dr. Michael J. Comb Receives CiteAb Lifetime Achievement Award
3D video animation of histone modifications and their effects on chromatin activation
If you contact CST for technical support, the same scientists who produced and validated your antibody will answer your call or email, sharing their experience and their data.