Register

Basket Updated

0

Items added

View Featured Offers >>
1% for the planet logo

Resources results for "CHD-1"

  • Products (22)
  • Resources (1469)

ATP-Dependent Chromatin Remodeling

Expert-reviewed interactive pathway providing a current overview of ATP-Dependent Chromatin Remodeling Complexes.

Chromo Protein Domain

A scientific resource for the Chromo protein domain containing information on structure, function, and binding to methylated lysine residues during chromatin remodeling.

LIM Protein Domain

A scientific resource for the LIM protein domain containing information on structure, function, and domain binding to mediate protein-protein interactions.

Protein Acetylation

Expert-reviewed interactive pathway providing a current overview of Protein Acetylation.

Emerging Therapeutic Approaches for Prostate Cancer

Roughly 1 in 8 men will be diagnosed with prostate cancer. So why do so many men lose their battle? Emerging therapeutic options may turn this around.

Epigenetics

Overview of epigenetic and chromatin regulation pathways, antibodies and related reagents, interactive pathway diagrams, and technical resources for epigenetics research.

ChIP Validated Antibodies and Primer Pairs

SimpleChIP Kits and Antibodies validated in-house by our antibody development scientists correlated to positive and negative control primers.

1% for the Planet Member

CST is a proud member of the 1% for the Planet organization, giving annually to benefit social and environmental causes.

PTMScan® Direct Cell Cycle & DNA Damage

Information and case study data for the PTMScan Direct Cell Cycle and DNA Damage Service offered by CST.

Cell Signaling Technology Joins 1% for the Planet

We have committed to donating 1% of our total annual revenue to environmental giving in collaboration with 1% for the Planet. Join the movement.

Cell Signaling Technology becomes 1% for the Planet Lighthouse Member

Cell Signaling Technology becomes 1% for the Planet Lighthouse Member

Resources

Our Calpain 1 Large Subunit (Mu-type) Antibody #2558 detects endogenous levels of total calpain 1 (large subunit) protein. The antibody detects full-length calpain 1 as well as calpain 1 autoproteolytically cleaved at leucine 28. The intensity of the cleaved band can vary depending on the species and type of cell lysate used.

Validated CST® Antibodies Work on the Cell DIVE Multiplexed Imaging Solution

Resources

We do not perform enzymatic activity assays internally using the Cell Lysis Buffer (10X) #9803, so we are unable to guarantee that the enzymatic activity will be conserved. We routinely use this buffer in immunoassays such as western immunoblot, IP, and ELISA without issue.

As a reference, the components of the Cell Lysis Buffer (10X) #9803 are provided below:
20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3VO4, 1 µg/ml leupeptin

Resources

When we validate an antibody for Simple Western™ we titrate the antibody dilution against a broad range of lysate concentrations. We recommend testing at least three antibody dilutions (1:10, 1:50 and 1:250) across a range of lysate concentrations (0.1 mg/mL – 1 mg/mL).

Lab Expectations listing page

The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench and share tips, tricks, and information.

Resources

The regions recognized by our PD-1 and PD-L1 antibodies are as follows:

PD-1 (EH33) Mouse mAb (IHC-Specific) #43248 binds the extracellular domain.
PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) #84651 binds the intracellular domain.
PD-1 (D4W2J) XP® Rabbit mAb #86163 binds the intracellular domain.
PD-L1 (E1L3N®) XP® Rabbit mAb #13684 binds the intracellular domain.
PD-L1 (Extracellular Domain Specific) (E1J2J) Rabbit mAb #15165 binds the extracellular domain.
PD-L1 (405.9A11) Mouse mAb #29122 binds the intracellular domain.
PD-L1 (D4H1Z) Rabbit mAb #60475 binds the extracellular domain.
PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) #64988 binds the intracellular domain.
PD-L1 (Extracellular Domain Specific) (D8T4X) Rabbit mAb #86744 binds the extracellular domain.

Resources

We typically recommend the PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 that comes with the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit #12581 as it has been validated for use with the kit. The PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 was made specifically for this assay kit and for ELISA formats. We have not tested any other lysis buffers with the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit #12581, so we cannot guarantee the performance. The ingredients of the PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 are as follows: 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 20 mM sodium Pyrophosphate, 25 mM Sodium Fluoride, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 ug/ml leupeptin.

Resources

CIP/Lambda Phosphatase Treatment of Cell Lysates:

1) Wash cells 2X with ice-cold PBS.

2) Add cold 1X cell lysis buffer that does not include phosphatase inhibitors [1-2 E7 cells per ml or 0.4ml per 10 cm plate (80-90% confluent)]. Our cell lysis buffer is prepared as follows:

20 mM Tris-HCl (pH 7.5)
150 mM NaCl
1 mM Na2EDTA
1 mM EGTA
1% Triton
1 ug/ml leupeptin
1mM PMSF

3) Add 20ul Quick CIP (NEB #M0525; https://www.neb.com/products/m0525-quick-cip#Product%20Information) per 400ul of cell extract.

4) Incubate with Quick CIP at 37C for 1 hour.

5) Add 20ul lambda protein phosphatase (CST

Hypoxia Signaling

Learn here about the HIF-1 signaling pathway and its impact on oxygen homeostasis and cellular adaptation. Click here.

Exceptional Specificity of XP® Monoclonal Antibodies

Case Study 1: Exceptional Specificity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.

Resources

Unfortunately, our PD-1 or PD-L1 antibodies have not been tested in neutralization assays. Therefore, we cannot guarantee their performance.


 

Resources

We observe multiple bands at or around 33-35 kDa in cells with high STING protein expression, such as THP-1 and HDLM-2. Regrettably, we are not certain about what the lower molecular weight band represents in the THP-1 extract. However, customers may be interested in the following possibilities:

1) Please see Wang, P. et al. (2018) Nucleic Acids Res. 46, 4054-4071 (PMID: 29547894; https://pubmed.ncbi.nlm.nih.gov/29547894/). The two bands in THP-1 extract may represent the alpha and beta isoforms of STING.

2) Please see Rodríguez-García, E. et al. (2018) Immunohorizons 2, 363-376 (PMID: 31026807; https://pubmed.ncbi.nlm.nih.gov/31026807/). STING isoforms 2 and 3 do not include #13647’s epitope. However, STING isoform 1 may be detected by #13647.

3) Given that the lower band is also observed in 293T cells transfected with a construct expressing human STING protein (see image on the product webpage; https://www.cellsignal.com//products/primary-antibodies/sting-d2p…

Protein and Pathway Abbreviations

Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.

Hypoxia and Cancer – the role of HIF-1α in oxygen sensing, metabolism, and tumorigenesis

Molecular oxygen is an essential element for metazoan life. Among its many roles, it functions as the final electron acceptor (oxidizing agent) during oxidative phosphorylation, a metabolic chain-reaction that generates energy in the form of ATP. HIF-1 alpha

Resources

The methods for the LC and MS are shown below:
 

 ParameterSetting
Sample prepIP sample resuspension volume20 uL
 Sample volume per injection4 uL
 

Better IHC Intro and Step 1: Antigen Retrieval

Stress Granule Life Cycle Diagram

Adhesion/ECM/Cytoskeleton

Overview of Adhesion and Extracellular Matrix signaling networks, antibodies and related reagents, interactive pathway diagrams, and technical resources.

Resources

Our protocol is as follows:

REAGENTS:
TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174
Lipopolysaccharides (LPS) #14011

DAY 1:

  1. Seed THP-1 cells at approximately 8 X 10^5 cells/mL in 15 cm plates with 20 ml RPMI +10% FBS + 0.05 mM 2-mercaptoethanol. (We use plates instead of flasks since the cells adhere to the plate after treatment with TPA).
  2. Treat plates overnight with 80nM TPA.

DAY 2:
  1. Wash treated plates with 10 mL 1x PBS, drain, and add fresh media (RPMI +10% FBS + 0.05 mM 2-mercaptoethanol).
  2. Allow cells to rest overnight.

DAY 3:
  1. Remove media from plates.
  2. Add 15 mL serum-free RPMI to plates.
  3. Treat plates with LPS at 1ug/mL for 8 hours.
  4. Collect media after 8 hours at centrifuge at 1500 - 2000 RCF for 5 minutes.
  5. While the media is spinning, wash the cells from the TPA/LPS treated plates with 1X PBS and lyse in 1X SDS/D…