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Resources results for "eIF3-alpha"

  • Products (278)
  • Resources (79)

Regulation of eIF2

Expert-reviewed interactive pathway providing a current overview of Regulation of eIF2.

RNA Regulation, Translational Control, and Protein Synthesis

Resources for the study of Translational Control, Protein Synthesis, and RNA Regulation including antibodies and associated reagents and interactive pathway diagrams.

Translational Control

Expert-reviewed interactive pathway providing a current overview of Translational Control.

Resources

Based on sequence homology, we predict that our α-Tubulin Antibody #2144 and α-Tubulin (11H10) Rabbit mAb #2125 will detect the alpha 1a, alpha 1b, alpha 1c, alpha 2, alpha 3c, alpha 3d, alpha 3e, alpha 4a, and alpha 4b subtypes. 

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Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 and Phospho-Akt (Ser473) (D9W9U) Mouse mAb #12694 detect over-expressed PKC alpha and beta. Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 also detects endogenous levels of PKC in Jurkat + CalA extract, but not in other endogenous models tested. Our Phospho-Akt (Ser473) (587F11) Mouse mAb #4051 detects over-expressed PKC alpha and beta but does not detect endogenous PKC in the models tested. Our Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058 does not detect over-expressed or endogenous PKC alpha and beta.

Regulation of eIF4E and p70 S6 Kinase

Expert-reviewed interactive pathway providing a current overview of Regulation of eIF4E and p70 S6K.

Resources

Our Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAbs #11818 and #42801 detect both phospho-ACC-alpha and phospho-ACC-beta. These antibodies recognize ACC-alpha only when phosphorylated at Ser80 in human, which corresponds to Ser79 in mouse and rat. They also recognize ACC-beta only when phosphorylated at Ser222 in human, corresponding to Ser212 in mouse and Ser218 in rat.

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From our testing, we have seen that our Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. It is possible that #12041 may detect other less common isoforms as well, but we have not confirmed this experimentally. Additionally, each of the isoforms that can be detected most likely have post-translational modifications that affect the molecular weight.

Our Glucocorticoid Receptor (D8H2) XP® Rabbit mAb #3660 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. However, based on sequence alignment (and unlike #12041) #3660 is predicted to cross-react with all known alternative translation start site generated isoforms of glucocorticoid receptor-α and glucocorticoid receptor-β (80 kDa).

Please refer to Uniprot ID: P04150 for the reference sequences of both alpha and beta isoforms of the Glucocorticoid Receptor. 

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Unfortunately, we are not aware if any of our amyloid-beta antibodies will detect the alpha-oligomer of the amyloid-beta protein.

Resources

PI4 Kinase Antibody #4902 detects endogenous levels of total PI4 Kinase isoform 230. In humans, PI4K230, PI4K92, and PI4K55 are four PI4 kinase isoforms that have been characterized and classified according to their molecular weights of 230, 92, and 55 kDa.  In the literature, alpha refers to the 230 kDa size band (UniProt ID: P42356) and beta refers to the 92 kDa size band (UniProt ID:Q9UBF8). Type 2 alpha (UniProt ID: Q9BTU6 ) and type 2 beta (UniProt ID: Q8TCG2) refer to the 55 kDa band.

m6A RNA Regulatory Diagram

Expert-reviewed diagram providing a current overview of the m6A RNA pathway with references to its role in tumor development

Unravelling Parkinson’s Disease: The Role of Mitophagy, Autophagy & Lysosomal Processing

PD is characterized by functional loss in dopaminergic neurons, believed to be caused by disruption of proteins involved in cell quality control pathways.

Adaptive Immune Response

Products and Related Resources for Adaptive Immune Response SARS-CoV-2 Research

About Us

Resources

We observe multiple bands at or around 33-35 kDa in cells with high STING protein expression, such as THP-1 and HDLM-2. Regrettably, we are not certain about what the lower molecular weight band represents in the THP-1 extract. However, customers may be interested in the following possibilities:

1) Please see Wang, P. et al. (2018) Nucleic Acids Res. 46, 4054-4071 (PMID: 29547894; https://pubmed.ncbi.nlm.nih.gov/29547894/). The two bands in THP-1 extract may represent the alpha and beta isoforms of STING.

2) Please see Rodríguez-García, E. et al. (2018) Immunohorizons 2, 363-376 (PMID: 31026807; https://pubmed.ncbi.nlm.nih.gov/31026807/). STING isoforms 2 and 3 do not include #13647’s epitope. However, STING isoform 1 may be detected by #13647.

3) Given that the lower band is also observed in 293T cells transfected with a construct expressing human STING protein (see image on the product webpage; https://www.cellsignal.com//products/primary-antibodies/sting-d2p…

Hypoxia and Cancer – the role of HIF-1α in oxygen sensing, metabolism, and tumorigenesis

Molecular oxygen is an essential element for metazoan life. Among its many roles, it functions as the final electron acceptor (oxidizing agent) during oxidative phosphorylation, a metabolic chain-reaction that generates energy in the form of ATP. HIF-1 alpha

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Wild type alpha-lytic protease (WaLP) is a serine endopeptidase that cleaves at the carboxyl terminal side of amino acids alanine, serine, threonine, and valine.

WaLP recognizes and cleaves SUMO proteins containing AGG, SGG, TGG or VGG motifs at the SUMO c-terminus.

One should verify that the predicted SUMO sequence from their model organism contains these motifs at the c-terminus.

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For imaging purposes, we recommend using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664 when working with rodent tissue.  We have noted non-specific labeling in specific sub-types of healthy cells in fixed-frozen mouse tissues (e.g. pancreatic alpha-cells) and nuclear background in rat samples with Cleaved Caspase-3 (Asp175) Antibody #9661 and Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579.

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Our Pan-Calcineurin A Antibody #2614 detects endogenous levels of the alpha isoform of Calcineurin A, however it may also recognize the beta and gamma isoforms. Regrettably, because it has been difficult to find suitable models, we have not specifically tested if this product will detect the beta and gamma isoforms. However, the antigenic peptide shares around 90% sequence identity with the beta isoform and around 80% sequence identity with the gamma isoform. Therefore, it is possible that there is cross-reactivity. This antibody does not cross-react with protein phosphatase 1 or 2A.

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In humans, there is a PTEN long isoform (isoform alpha) that can potentially be detected by our PTEN (D4.3) XP Rabbit mAb #9188 and PTEN (138G6) Rabbit mAb #9559 based on sequence homology. There is also a paper that cites #9559 specifically detecting the long isoform of PTEN. [see H. Liang et. al (2014) Cell Metab. 19, 836-48, PMID: 24768297, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097321]

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Unfortunately, we have not performed experiments in-house to determine if any of our α-Synuclein antibodies can detect oligomers or fibrils of α-Synuclein. We have found a helpful publication in which our α-Synuclein (D37A6) XP® Rabbit mAb #4179 is used to detect oligomers (PMID: 24898419, Defined α-synuclein prion-like molecular assemblies spreading in cell culture by Aulić, S. et al.).
 

Glutamine Metabolism

Expert-reviewed interactive pathway providing a current overview of Glutamine Metabolism signaling pathway.

Neuroinflammation in Neurodegenerative Diseases

Neuroinflammation is the activation of an immune response in the CNS by the microglia and astrocytes. While not linked mechanistically to neurodegenerative diseases, neuroinflammation is associated with the progression of Alzheimer’s disease, Parkinson’s

Neuroinflammation in Neurodegenerative Diseases

Chronic neuroinflammation is associated with neurodegenerative diseases like Alzheimer’s, Parkinson’s, amyotrophic lateral sclerosis, and others.

Adhesion/ECM/Cytoskeleton

Overview of Adhesion and Extracellular Matrix signaling networks, antibodies and related reagents, interactive pathway diagrams, and technical resources.

Viral Entry, Replication, Transcription, and Translation

Products and Related Resources for Viral Entry, Replication, and Transcription SARS-CoV-2 Research

Contribution of Extracellular Matrix to EMT

Expert-reviewed interactive pathway providing a current overview of Contribution of Extracellular Matrix to EMT Signaling.

Using SignalScan to Investigate Infection and Immune Response

SignalScan bridges the gap between PCR-based detection of COVID-19 infection and uncovering the molecular signature of infection and host immune response.

Neuronal and Glial Cell Markers

Expert-reviewed interactive diagrams providing current overviews of neuronal and glial cell markers, as well as links to products from CST.

Neural and Glial Cell Markers

There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.