Can I combine antibodies used in a SignalStar™ assay with direct conjugates?

SignalStar™ Multiplex IHC kits and reagents have not been validated for use in combination with direct conjugates. It is likely possible to incorporate direct conjugates into your protocol, however, because the SignalStar reagents benefit from fluorescent signal amplification, there may be spectral bleed-through that results from using the assay with conjugates that are not amplified.

Last updated: August 25, 2023