Can I combine antibodies used in a SignalStar™ assay with direct conjugates?

SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit #32722 enables you to remove the fluorescent oligos after SignalStar mIHC in order to stain and visualize direct immunofluorescence. We have found that many direct conjugates against strong cell surface markers work well when used in this manner. Please see our recent poster for more information. In addition, please see our line of directly conjugated antibodies for use in direct immunofluorescence imaging.

Last updated: September 12, 2024