ER-phagy Cargo Receptor Antibody Sampler Kit #82484
Product Information
Kit Usage Information
Protocols
Product Description
The ER-phagy Cargo Receptor Antibody Sampler Kit provides an economical means of detecting proteins functioning as ER-phagy cargo receptors. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Background
ER-phagy is one of the key processes that regulates ER morphology and functions in the fragmentation and removal of ER segments. Accumulation of unfolded proteins or exposure to conditions such as metabolic or oxidative stress leads to compensatory ER stress programs that remodel the ER structure and activate signaling pathways to cope with those challenges. Activation of ER stress pathways, such as the unfolded protein response (UPR), increases ER membrane to help manage increased demand. Once the stress conditions subside, ER-phagy can eliminate unneeded ER fragments (reviewed in 1-3). Over the last several years, there have been advances in our understanding of ER-phagy. A significant advancement is the discovery of several ER-resident autophagy receptors, including FAM134B, CCPG1, ATL3, TEX264, SEC62, and RTN3L. These cargo receptors have distinct modes of regulation, expression patterns, and localization within the ER. Each of these proteins contains at least one LIR or GIM, which facilitates binding to LC3 or GABARAP family members on the autophagosome. These autophagy receptors contribute to ER-phagy at specific sites on the ER and in response to different stimuli, including nutrient deprivation, ER stress, and changes in calcium. Loss of these proteins is associated with damaged ER expansion, sustained activation of ER stress pathways, and pathophysiological regulation.
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