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90298
Mouse Microglia Marker IF Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit
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Mouse Microglia Marker IF Antibody Sampler Kit #90298

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Mouse Microglia Marker IF Antibody Sampler Kit: Image 1
Confocal immunofluorescent analysis of mouse subiculum using CD68 (E3O7V) Rabbit mAb (red). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to colabeling with GFAP (GA5) Mouse mAb (Alexa Fluor® 488 Conjugate) #3655 (right, green), β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #42284 (right, cyan pseudocolor), and DAPI #4083 (right, blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 2
Confocal immunofluorescent analysis of mouse subiculum from the 5XFAD mouse model of Alzheimer's disease using CD68 (E3O7V) Rabbit mAb (red). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to colabeling with GFAP (GA5) Mouse mAb (Alexa Fluor® 488 Conjugate) #3655 (right, green), β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #42284 (right, cyan pseudocolor), and DAPI #4083 (right, blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 3
Flow cytometric analysis of RAW 264.7 cells, untreated (blue) or treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (100 ng/mL, 24 h; green) using CD68 (E3O7V) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 4
Western blot analysis of extracts from various cell lines using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 5
Western blot analysis of extracts from various cell lines using F4/80 (D4C8V) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 6
Western blot analysis of cell extracts from Baf3, 32D, and mouse spleen using HS1 (D5A9) XP® Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 7
Confocal immunofluorescent analysis of mouse brain microglia (left) or peripheral macrophages of the liver (right) using CD11b/ITGAM (M1/70) Rat mAb (green). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 8
Confocal immunofluorescent analysis of brain from an amyloid mouse model of Alzheimer's Disease using CD45 (30-F11) Rat mAb (green) and β-Amyloid (D54D2) XP® Rabbit mAb #8243 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 9
Western blot analysis of extracts from J774A.1 and Raw 264.7 cells using ASC/TMS1 (D2W8U) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 10
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 11
Confocal immunofluorescent analysis of wild-type mouse brain (left) and liver (right) using TMEM119 (E3E1O) Rabbit mAb (green). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 12
Confocal immunofluorescent analysis of the ventricular zone in P21 mouse brain using Ki-67 (D3B5) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 13
Western blot analysis of extracts from various mouse cells using CD68 (E3O7V) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 14
Immunoprecipitation of Iba1/AIF-1 from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Iba1/AIF-1 (E4O4W) XP® Rabbit mAb. Western blot analysis was performed using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 15
Immunoprecipitation of F4/80 from RAW 264.7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is F4/80 (D4C8V) XP® Rabbit mAb. Western blot analysis was performed using F4/80 (D4C8V) XP® Rabbit mAb as the primary antibody and Mouse Anti-Rabbit IgG (Conformation Specific) (L29A9) mAb #3678, followed by Anti-mouse IgG, HRP-linked Antibody #7076 as the secondary antibodies.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 16
Immunohistochemical analysis of paraffin-embedded mouse spleen using HS1 (D5A9) XP® Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 17
Confocal immunofluorescent analysis of brain from an amyloid mouse model of Alzheimer’s disease using CD11b/ITGAM (M1/70) Rat mAb (green) and β-Amyloid (D54D2) XP® Rabbit mAb #8243 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 18
Confocal immunofluorescent analysis of mouse brain microglia (left) or peripheral macrophages of the liver (right) using CD45 (30-F11) Rat mAb (green). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 19
Immunoprecipitation of ASC/TMS1 from J774A.1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ASC (D2W8U) Rabbit mAb. Western blot analysis was performed using ASC/TMS1 (D2W8U) Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 20
Confocal immunofluorescent analysis of brain from an amyloid mouse model of Alzheimer’s disease. Sections were labeled with TMEM119 (E3E1O) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (yellow). Plaques were then stained with β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #42284 (red) after blocking free secondary binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900. Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 21
Confocal immunofluorescent analysis of HeLa cells using Ki-67 (D3B5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 22
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse CD68 protein (mCD68-Myc/DDK; +), using CD68 (E3O7V) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 23
Immunohistochemical analysis of paraffin-embedded human neuroendocrine carcinoma of the lung using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 24
Confocal immunofluorescent analysis of Tg2576 brain, which overexpresses mutant human APP695. Sections were labeled with F4/80 (D4C8V) XP® Rabbit mAb (green), β-Amyloid (D3D2N) Mouse mAb #15126 (yellow), and GFAP (E6N9L) Mouse mAb #34001 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 25
Immunohistochemical analysis of paraffin-embedded LL2 syngeneic tumor using HS1 (D5A9) XP® Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 26
Immunohistochemical analysis of paraffin-embedded J774A.1 cell pellet (left, positive) or RAW 264.7 cell pellet (right, negative) using ASC/TMS1 (D2W8U) Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 27
Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 28
Immunohistochemical analysis of paraffin-embedded mouse colon using CD68 (E3O7V) Rabbit mAb performed on the Leica® BOND™ Rx.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 29
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 30
Confocal immunofluorescent analysis of normal mouse spleen using F4/80 (D4C8V) XP® Rabbit mAb (red) and B220/CD45R Alexa Fluor® 488 conjugate (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 31
Confocal immunofluorescent analysis of mouse Tg2576 brain which overexpresses mutant human APP695. Sections were first labeled with HS1 (D5A9) XP® Rabbit mAb (green) and APP/β-Amyloid (NAB228) Mouse mAb #2450 (yellow). After blocking free secondary binding sites with Mouse (G3A1) mAb IgG1 Isotype Control #5415, sections were incubated with GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (red). Nuclei were labeled with Hoechst 33342 #4082 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 32
Immunohistochemical analysis of paraffin-embedded mouse forestomach using ASC/TMS1 (D2W8U) Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 33
Immunohistochemical analysis of paraffin-embedded mouse lung using CD68 (E3O7V) Rabbit mAb performed on the Leica® BOND™ Rx.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 34
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (left) or Iba1/AIF-1 (E5N4J) Mouse mAb (IHC Formulated) #58970 (right) performed on the Leica® BOND™ Rx. These two antibodies detect independent, unique epitopes on human Iba1/AIF-1 protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 35
Confocal immunofluorescent analysis of RAW 264.7 cells (left, positive) or Neuro-2a cells (right, negative), using F4/80 (D4C8V) XP® Rabbit mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 36
Confocal immunofluorescent analysis of 32D cells (left) and C2C12 cells (right), using HS1 (D5A9) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 37
Immunohistochemical analysis of paraffin-embedded mouse brain using ASC/TMS1 (D2W8U) Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 38
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD68 (E3O7V) Rabbit mAb performed on the Leica® BOND™ Rx.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 39
Immunohistochemical analysis of paraffin-embedded mouse brain using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 40
Flow cytometric analysis of NIH/3T3 cells (red) and 32D cells (blue), using HS1 (D5A9) XP® Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 41
Immunohistochemical analysis of paraffin-embedded mouse colon using ASC/TMS1 (D2W8U) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 42
Immunohistochemical analysis of paraffin-embedded mouse testis using CD68 (E3O7V) Rabbit mAb performed on the Leica® BOND™ Rx.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 43
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 44
Immunohistochemical analysis of paraffin-embedded mouse thymus using ASC/TMS1 (D2W8U) Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 45
Immunohistochemical analysis of paraffin-embedded RAW 264.7 cell pellet (left, positive), Renca cell pellet (middle, positive), or Neuro-2a cell pellet (right, negative) using CD68 (E3O7V) Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 46
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 47
Immunohistochemical analysis of paraffin-embedded mouse small intestine using ASC/TMS1 (D2W8U) Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 48
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD68 (E3O7V) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 49
Immunohistochemical analysis of paraffin-embedded mouse small intestine using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 50
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor (top left), 4T1 syngeneic mammary tumor (top right), Renca cell pellet (bottom left), and 4T1 cell pellet (bottom right) using ASC/TMS1 (D2W8U) Rabbit mAb. Both tumors show staining of infiltrating immune cells. Note the presence of staining in the Renca tumor cells and the lack of staining in the 4T1 tumor cells consistent with staining results on corresponding cell pellets.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 51
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using CD68 (E3O7V) Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 52
Immunohistochemical analysis of paraffin-embedded mouse spleen using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 53
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using CD68 (E3O7V) Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 54
Dual immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (brown) and APP/β-Amyloid (NAB228) Mouse mAb #2450 (red).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 55
Immunohistochemical analysis of paraffin-embedded mouse liver using CD68 (E3O7V) Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 56
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 57
Confocal immunofluorescent analysis of mouse Tg2576 brain which overexpresses mutant human APP695. Sections were first labeled with ASC/TMS1 (D2W8U) Rabbit mAb #67824 (green) and APP/β-Amyloid (NAB228) Mouse mAb #2450 (yellow). After blocking free secondary binding sites with Mouse (G3A1) mAb IgG1 Isotype Control #5415, sections were incubated with GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (red). Nuclei were labeled with Hoechst 33342 #4082 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 58
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using CD68 (E3O7V) Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 59
Immunohistochemical analysis of paraffin-embedded THP-1 cell pellet (left, positive) or SH-SY5Y cell pellet (right, negative) using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 60
Confocal immunofluorescent analysis of human cortex (left) and mouse CA1 hippocampus (right) using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (green). In mouse tissue sections, cell nuclei were labeled with DAPI (blue). Images kindly provided by Dr. Simone Brioschi and Dr. Marco Colonna (Washington University) and used with permission.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 61
Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) either untreated (upper left) or treated with LPS (50 ng/ml, 4 hr, middle) or LPS followed by ATP (5 mM, 45 min, upper right), and J774A.1 (lower left) or Raw 264.7 (lower right) cells, using ASC/TMS1 (D2W8U) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note the translocation of ASC to inflammasomes following stimulation with LPS and ATP (white arrows).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 62
Confocal immunofluorescent analysis of mouse colon (left) and liver (right) using CD68 (E3O7V) Rabbit mAb (red). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) (Alexa Fluor® 488 Conjugate) #68206 (green). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 63
Confocal immunofluorescent analysis of mouse small intestine using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 64
Flow cytometric analysis of Raw264.7 cells (blue) and J774A.1 cells (green) using ASC/TMS1 (D2W8U) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Mouse Microglia Marker IF Antibody Sampler Kit: Image 65
Confocal immunofluorescent analysis of RAW 264.7 cells (left, positive) and Neuro-2a cells (right, negative) using CD68 (E3O7V) Rabbit mAb (green). Cells were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 66
Confocal immunofluorescent analysis of mouse liver using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 67
Confocal immunofluorescent analysis of microglia in mouse hippocampus using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (green). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 68
Confocal immunofluorescent analysis of THP-1 cells differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, 24 hr; left, positive) and SH-SY5Y cells (right, negative), using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Microglia Marker IF Antibody Sampler Kit: Image 69
Flow cytometric analysis of SH-SY5Y cells (blue, negative) and THP-1 cells (green, positive) using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
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To Purchase # 90298
Cat. # Size Qty. Price
90298T		 1 Kit  (9 x 20 microliters)
$ 708

Custom Formulation

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Iba1/AIF-1 (E4O4W) XP® Rabbit mAb 17198 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
 H M R 17 Rabbit IgG
TMEM119 (E3E1O) Rabbit mAb 90840 20 µl
  • IF
 M Rabbit IgG
ASC/TMS1 (D2W8U) Rabbit mAb 67824 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
 M 22 Rabbit IgG
HS1 (D5A9) XP® Rabbit mAb 3892 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
 M R 80 Rabbit IgG
CD11b/ITGAM (M1/70) Rat mAb 46512 20 µl
  • IF
 M Rat IgG2b kappa
CD45 (30-F11) Rat mAb 55307 20 µl
  • IF
 M Rat IgG2b kappa
F4/80 (D4C8V) XP® Rabbit mAb 30325 20 µl
  • WB
  • IP
  • IF
 M 65-250 Rabbit IgG
Ki-67 (D3B5) Rabbit mAb 9129 20 µl
  • IF
  • F
 H M R 359 Rabbit IgG
CD68 (E3O7V) Rabbit mAb 97778 20 µl
  • WB
  • IHC
  • IF
  • F
 M 70-80, 130-140, 200 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
 Rab Goat 

Product Description

The Mouse Microglia Marker IF Antibody Sampler Kit provides an economical means of detecting proteins identified as microglia markers by immunofluorescence and/or western blot. This kit includes enough primary antibodies to perform at least twenty IF-F tests or two western blot experiments per primary antibody.

Specificity / Sensitivity

Each antibody in the Mouse Microglia Marker IF Antibody Sampler Kit detects endogenous levels of its target protein. HS1 has a calculated size of 54 kDa, but has an apparent molecular weight of 80 kDa on SDS-PAGE gels. CD45 (30-F11) Rat mAb and CD11b/ITGAM (M1/70) Rat mAb detect an epitope within the extracellular domain. CD68 (E3O7V) Rabbit mAb has shown staining of uncertain specificity in mouse endometrial epithelium by immunohistochemistry.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ala139 of human Iba1/AIF-1 protein, Leu310 of mouse HS1 protein, Gln260 of mouse F4/80 protein, the amino terminus of human TMEM119 and Ki-67 proteins, and recombinant mouse ASC/TMS1 protein and mouse CD68 that reacts with an epitope surrounding Gly113. CD11b/ITGAM (M1/70) Rat mAb and CD45 (30-F11) Rat mAb were purified from tissue culture supernatant via affinity chromatography.

Background

Microglia are the resident macrophages of the central nervous system, responsible for immune response and maintenance of CNS homeostasis (1). Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammatory factor 1 (AIF-1), is uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia/macrophages in the brain and other tissue (2,3). Transmembrane protein 119 (TMEM119) is a cell-surface protein of unknown function, expressed exclusively by the microglia subset of myeloid and neural cells (4). Iba1+ microglia with both ramified and amoeboid morphologies express TMEM119, while Iba1+ macrophages are TMEM119 negative (5). TMEM119 and other homeostatic genes have been shown to be downregulated in disease-associated microglia (DAM) (6). Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (7). F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (8-10). Expression of F4/80 has been observed in microglia and subset populations of dendritic cells (11). The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein expressed in all nucleated hematopoietic cells (12). Studies suggest CD45 plays a role in regulation of microglial activation (13,14). CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (15,16). It localizes to the lysosomal membrane and is upregulated during microglial activation (17,18). Ki-67 is a nuclear nonhistone protein (19) universally expressed among proliferating cells and absent in quiescent cells (20). Previous work identifying markers of specific brain cell types using RNA-seq has shown HS1 and ASC/TMS1 to be useful and specific tools to study microglia (21). HS1 is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (22) and ASC/TMS1 has been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (23).
  1. Ginhoux, F. and Prinz, M. (2015) Cold Spring Harb Perspect Biol 7, a020537.
  2. Deininger, M.H. et al. (2002) FEBS Lett 514, 115-21.
  3. Ito, D. et al. (1998) Brain Res Mol Brain Res 57, 1-9.
  4. Bennett, M.L. et al. (2016) Proc Natl Acad Sci U S A 113, E1738-46.
  5. Satoh, J. et al. (2016) Neuropathology 36, 39-49.
  6. Deczkowska, A. et al. (2018) Cell 173, 1073-81.
  7. Murray, P.J. and Wynn, T.A. (2011) Nat Rev Immunol 11, 723-37.
  8. Hirsch, S. et al. (1981) J Exp Med 154, 713-25.
  9. Austyn, J.M. and Gordon, S. (1981) Eur J Immunol 11, 805-15.
  10. McKnight, A.J. et al. (1996) J Biol Chem 271, 486-9.
  11. Greter, M. et al. (2015) Front Immunol 6, 249.
  12. Huntington, N.D. and Tarlinton, D.M. (2004) Immunol Lett 94, 167-74.
  13. Tan, J. et al. (2000) J Biol Chem 275, 37224-31.
  14. Cosenza-Nashat, M.A. et al. (2006) Brain Pathol 16, 256-65.
  15. Rabinowitz, S.S. and Gordon, S. (1991) J Exp Med 174, 827-36.
  16. Holness, C.L. and Simmons, D.L. (1993) Blood 81, 1607-13.
  17. Wong, A.M. et al. (2005) Neurosci Lett 390, 76-80.
  18. Hendrickx, D.A.E. et al. (2017) J Neuroimmunol 309, 12-22.
  19. Mincheva, A. et al. (1994) Cytogenet Cell Genet 65, 276-7.
  20. Doorn, K.J. et al. (2014) Neural Plast 2014, 959154.
  21. Zhang, Y. et al. (2014) J Neurosci 34, 11929-47.
  22. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  23. Srinivasula, S.M. et al. (2002) J Biol Chem 277, 21119-22.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
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