Name: Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit
Price: 645.00 USD
Availability: InStock

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Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 1 — Western blot analysis of extracts from COS-7 and HUVEC cells using PAI-1 (D9C4) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 2 — Western blot analysis of 1 ng recombinant Human Interleukin-6 (hIL-6) #8904 using IL-6 (D3K2N) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 3 — Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/ml, 3 hr; +), using IL-1β (D3U3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 4 — Western blot analysis of extracts from various cell lines using MMP-3 (D7F5B) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 5 — Western blot analysis of recombinant human CXCL10 protein using CXCL10 (D5L5L) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 6 — Western blot analysis of extracts from differentiated THP-1 cells, untreated or treated overnight with LPS, using CCL5/RANTES (R40) Antibody.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 7 — Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing human MCP-1 protein (hMCP-1; +), using MCP-1 Antibody (Carboxy-terminal Antigen) (upper) or β-actin (D6A8) Rabbit mab #8457 (lower).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 8 — Western blot analysis of extracts from the media of THP-1 cells differentiated with TPA #4174 (80 nM; overnight), with or without LPS (1 μg/ml; overnight), using TNF-α (D5G9) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 9 — After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO^® is added and emits light during enzyme catalyzed decomposition.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 10 — Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight), untreated (-) or LPS-treated (100 ng/ml, 6 hr; +) and treated with Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation; +), using IL-6 (D3K2N) Rabbit mAb or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 11 — Western blot analysis of recombinant Human Interleukin-1β (hIL-1β) #8900 using IL-1β (D3U3E) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 12 — Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (50 ng/ml, 16 hr; +), using CXCL10 (D5L5L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 13 — Western blot analysis of HeLa cells, mock transfected or transfected with human CCL5/RANTES construct, using CCL5/RANTES (R40) Antibody.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 14 — Western blot analysis of extracts from A549 cells, untreated (-) or treated with TNF-α (20 ng/ml, 6 hr; +), using MCP-1 Antibody (Carboxy-terminal Antigen) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 15 — Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM; overnight), with or without LPS (1 μg/ml; various time points), using TNF-α (D5G9) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 16 — Immunoprecipitation of IL-6 from NCI-H460 cell extracts, using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or IL-6 (D3K2N) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IL-6 (D3K2N) Rabbit mAb.

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 17 — Confocal immunofluorescent analysis of THP-1 cells, untreated (left) or LPS-treated (500 ng/ml, 2 hr; right), using IL-1β (D3U3E) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 18 — Immunoprecipitation of MCP-1 protein from conditioned medium of A549 cells treated with TNF-α (20 ng/ml, 6 hr). Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is MCP-1 Antibody (Carboxy-terminal Antigen). Western blot analysis was performed using MCP-1 Antibody (Carboxy-terminal Antigen).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 19 — Western blot analysis of recombinant human TNF-α #8902 using TNF-α (D5G9) Rabbit mAb (left) or TNF-α Antibody #3707 (right).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 20 — Flow cytometric analysis of THP-1, untreated (blue, negative) or treated with LPS #14011 (100 ng/ml, 3 hr; green, positive) using IL-1β (D3U3E) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

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To Purchase # 38461

Cat. #	Size	Qty.	Price
38461T	1 Kit (8 x 20 microliters)		$ 645

Product Includes
Related Products

Product Includes	Quantity	Applications	Reactivity	MW(kDa)	Isotype
IL-1β (D3U3E) Rabbit mAb 12703	20 µl	WB IF F	H	17, 31	Rabbit IgG
CCL5/RANTES (R40) Antibody 2987	20 µl	WB	H	10	Rabbit
CXCL10 (D5L5L) Rabbit mAb 14969	20 µl	WB	H	10	Rabbit IgG
PAI-1 (D9C4) Rabbit mAb 11907	20 µl	WB	H Mk B	48	Rabbit IgG
IL-6 (D3K2N) Rabbit mAb 12153	20 µl	WB IP	H	21-28	Rabbit IgG
TNF-α (D5G9) Rabbit mAb 6945	20 µl	WB IP	H	18, 25	Rabbit IgG
MMP-3 (D7F5B) Rabbit mAb 14351	20 µl	WB	H R	60	Rabbit IgG
MCP-1 Antibody (Carboxy-terminal Antigen) 39091	20 µl	WB IP	H	13-15	Rabbit
Anti-rabbit IgG, HRP-linked Antibody 7074	100 µl	WB	Rab		Goat

Kit Usage Information

Protocols

2987: Western Blotting
6945: Western Blotting, Immunoprecipitation (Agarose)
7074: Western Blotting
11907: Western Blotting
12153: Western Blotting, Immunoprecipitation (Magnetic)
12703: Western Blotting, Immunofluorescence, Flow
14351: Western Blotting
14969: Western Blotting
39091: Western Blotting, Immunoprecipitation (Magnetic)

Product Description

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides an economical means of detecting multiple components of the SASP. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit detects endogenous levels of its target protein.
CXCL10 (D5L5L) Rabbit mAb also cross-reacts with a 100kDa protein of unknown origin in some cell lines.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Arg294 of human PAI-1 protein, residues surrounding Ser417 of human MMP3 protein, recombinant human IL-1β protein, recombinant human CXCL10 protein, recombinant human IL-6 protein, and recombinant human TNF-α protein.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg40 of human RANTES and near the carboxy terminus of human MCP-1 protein.

Background

Senescence is characterized by stable stress-induced proliferative arrest and resistance to mitogenic stimuli, as well as the secretion of proteins such as cytokines, growth factors and proteases. These secreted proteins comprise the senescence-associated secretory phenotype (SASP). Senescent cells are thought to accumulate as an organism ages, and contribute to age-related diseases, including cancer, through promotion of inflammation and disruption of normal cellular function (1,2). The composition of the SASP varies, and SASP components can be either beneficial or deleterious in human disease, depending on the context (3).
Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides a collection of antibodies to various SASP components, including TNF-alpha, interleukin-6 (IL-6), the multifunctional cytokine IL-1beta, the chemokines CXCL10, RANTES/CCL5 and MCP-1, the matrix metalloprotease MMP3, and the serine-protease inhibitor PAI-1.

Pathways

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Database Links

UniProt ID: P01584 , P05231 , P13501 , P08254 , P05121 , P02778 , P13500 , P01375

Entrez-Gene Id: 3553 , 3569 , 6352 , 4314 , 5054 , 3627 , 6347 , 7124

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Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit #38461