View Featured Offers >>
81229
TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

TIM-3 (D5D5R) XP® Rabbit mAb (BSA and Azide Free) #81229

Reviews ()
Citations (0)
Filter:
  1. WB
  2. IHC
  3. F
Western Blotting Image 1: TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing full-length human Myc/DDK-tagged TIM-3 (hTIM-3-Myc/DDK; +), using TIM-3 (D5D5R) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Western Blotting Image 2: TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from primary human CD4+ T cells and various cell lines using TIM-3 (D5D5R) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded renal clear cell carcinoma using TIM-3 (D5D5R) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TIM-3 (D5D5R) XP® Rabbit mAb. Note staining of alveolar macrophages. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TIM-3 (D5D5R) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human tonsil using TIM-3 (D5D5R) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma usng TIM-3 (D5D5R) XP® Rabbit mAb (yellow), PD-1 (D4W2J) XP® Rabbit mAb #86163 (green), PD-L1 (E1L3N®) XP® Rabbit mAb #13684 (red), LAG3 (D2G4O) XP® Rabbit mAb #15372 (magenta), CD8α (C8/144B) Mouse mAb #70306 (orange), and Pan-keratin (C11) Mouse mAb #4545 (cyan). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using TIM-3 (D5D5R) XP® Rabbit mAb (yellow), PD-1 (EH33) Mouse mAb #43248 (green), CD8α (C8/144B) Mouse mAb #70306 (magenta), CD68 (D4B9C) XP® Rabbit mAb #76437 (red), Pan-keratin (C11) Mouse mAb #4545 (cyan), and LAG3 (D2G4O) XP® Rabbit mAb #15372 (orange). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using TIM-3 (D5D5R) XP® Rabbit mAb (yellow), PD-1 (D4W2J) XP® Rabbit mAb #86163 (green), B7-H3 (D9M2L) XP® Rabbit mAb #14058 (red), B7-H4 (D1M8I) XP® Rabbit mAb (cyan), LAG3 (D2G4O) XP® Rabbit mAb #15372 (magenta), and VISTA (D1L2G) XP® Rabbit mAb #64953 (orange). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded cell pellets, primary CD4+ T cells (left) and HT-29 cells (right), using TIM-3 (D5D5R) XP® Rabbit mAb. CD4+ T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml). Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free)
Flow cytometric analysis of Jurkat cells (blue) and primary CD4+ T cells (green) using TIM-3 (D5D5R) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) #8907 (6.7 ng/ml). Data were generated using the standard formulation of this product.
To Purchase # 81229
Cat. # Size Qty. Price
81229SF
100 µg
$ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 45-70
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #45208. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #45208.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

TIM-3 (D5D5R™) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total TIM-3 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human TIM-3 protein.

Background

T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation (1-3 ). TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer (4,5), and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype (5). Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells (6). Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced anti-tumor responses in a mouse model of colorectal cancer (7). In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease (1). Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death (8).
  1. Monney, L. et al. (2002) Nature 415, 536-41.
  2. Sánchez-Fueyo, A. et al. (2003) Nat Immunol 4, 1093-101.
  3. Sabatos, C.A. et al. (2003) Nat Immunol 4, 1102-10.
  4. Jones, R.B. et al. (2008) J Exp Med 205, 2763-79.
  5. Sakuishi, K. et al. (2010) J Exp Med 207, 2187-94.
  6. Chiba, S. et al. (2012) Nat Immunol 13, 832-42.
  7. Huang, Y.H. et al. (2015) Nature 517, 386-90.
  8. Zhu, C. et al. (2005) Nat Immunol 6, 1245-52.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
D5D5R is a trademark of Cell Signaling Technology. Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.