Resources results for "cd19"
- Products (306)
- Resources (70)
Resources
The CD44 gene encodes 20 exons. Alternative splicing gives rise to CD44s and multiple CD44 variants (CD44v). CD44s is encoded by constant exons 1-5, 16-18, and 20. The CD44 variants are produced by the insertion of variant exons 6–15, typically identified as v1–v10. The CD44 variants can contain one or more variant regions, such as CD44v3, CD44v6, CD44v4–10, or CD44v8–10. Exon 19 is spliced out in all forms of CD44.
Our CD44 (156-3C11) Mouse mAb #3570 recognizes residues surrounding Pro210 of human CD44 (UniProt ID #P16070; https://www.uniprot.org/uniprot/P16070), which are encoded by exon 5. Therefore, #3570 is predicted to recognize all variants of human CD44.
Resources
Our CD45 (D9M8I) XP (#13917), CD45 (D4H7K) ( #72787), and CD45 (D3F8Q) (#70257) Rabbit mAbs recognize intracellular (cytoplasmic) epitopes in the human (UnitProt ID P08575) and mouse (UniProt ID P06800) CD45 proteins, respectively. The CD45 isoforms differ in their extracellular domains as a result of alternative splicing. Therefore, #13917, #70257, and #72787 are predicted to detect all of the CD45 isoforms including CD45RO and CD45RA.
Resources
We prepare Jurkat -/+ Anti-CD3 antibody (10 µg/mL for 2 min) cell extracts as follows:
- Jurkat cells are cultured to the desired volume (200-400 mL) and a density of approximately 1x10^6 cells/mL, then serum-starved overnight.
- The next day, half the Jurkat cells are collected, pelleted by centrifugation, and resuspended in 20mL serum-free RPMI 1640 media in a 50mL conical tube before treating with anti-CD3 antibody. The media volume is reduced to conserve the amount of antibody reagent required to treat the cells at a concentration of 10 µg/mL.
- The cells are treated with 10 µg/mL Human CD3ε Activating (OKT3) Mouse mAb (Low Endotoxin, Azide-free) (#92511; https://www.cellsignal.com/produc…