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"Mouse on mouse" (MOM) background is caused by the anti-mouse secondary antibody binding to endogenous mouse IgG expressed in the tissue resulting in non-specific staining, which can complicate the interpretation of your results. A secondary-only control should be included to assess the potential amount of MOM background in your samples. There are commercially available kits and blocking solutions available that are designed to enable the use of mouse antibodies on mouse tissue. These include M.O.M.® (Mouse on Mouse) Blocking Reagent from Vector Labs and UltraVision™ Quanto Mouse on Mouse from Thermo. We do not routinely use these products in-house so we are not able to recommend one over another.
Yes, our CaMKII (pan) (D11A10) Rabbit mAb #4436 detects the delta isoform.
Yes, you can use BSA #9998 as a carrier for cytokines. It should be dissolved into 1X PBS and sterile filtered.
Cell viability assays measure the population of live, viable cells within a sample. Typically, viability assays measure markers of cell health, including cellular metabolism, ATP levels, and cell proliferation.
We have had customers perform this assay using frozen cell pellets, prior to adding any fractionation buffer, and the subsequent fractionation worked just fine. Once you start fractionating, we recommend following the protocol through to the end. We do not recommend leaving the cell pellets frozen for a prolonged period of time because, even frozen, proteases and phosphatases can slowly degrade the sample. However, leaving the frozen pellet over a weekend or a few days should be ok. Results may vary depending on the stability of your specific targets of interest.
SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be possible to perform other assays following SignalStar, such as hematoxylin and eosin (H&E) staining.
Yes. In our protocol, we recommend a probe-based sonicator because it is less expensive and widely used in many labs. However, you can use a Covaris water bath sonicator. You will still need to optimize the sonication program based on your DNA image, since you want to avoid over-sonicating when performing sonication-ChIP as this can damage chromatin quality/integrity. When looking at sonication results, chromatin DNA fragments should appear as a smear typically ranging between 200 to 1000 bp in size. We recommend you use the condition that requires the minimal sonication cycles, as long as the majority of DNA is less than 1000bp.
作为指导方针,本次讲座将与您分享CST流式细胞术专家在日常的试验工作中经常使用的经验和技巧。
Why are antigen retrieval and antibody diluent important for the immunohistochemistry protocol? Our IHC expert offers protocol advice.
Regrettably, we cannot offer any firm guarantees when glycerol formulated antibodies have been frozen. However, we have performed stability testing by western blot for a representative group of rabbit polyclonal, rabbit monoclonal, and mouse monoclonal antibodies, and found that two freeze/thaw cycles on dry ice (-78.5C) did not affect their activity.
The antigens for our COX IV Antibody #4844 and COX IV (3E11) Rabbit mAb #4850 are 100% conserved with COX IV isoform 1. There is less than 20% homology of these antigens with COX IV isoform 2, therefore we do not expect these antibodies to detect isoform 2.The antigen for our COX IV (4D11-B3-E8) Mouse mAb #11967 is also 100% conserved with COX IV isoform 1. However, the antigen used to produce #11967 is 79% homologous with COX IV isoform 2. Therefore there may be cross-reactivity of this antibody with isoform 2, but we have not tested this in-house and cannot guarantee it.
本讲座将为大家系统地介绍cGAS-STING信号通路在天然免疫和肿瘤免疫研究领域的广泛作用,以及作为干预靶标的巨大潜力。
Apoptosis is a type of programmed cell death that occurs normally throughout the lifespan and can also occur in response to harmful stimuli. Apoptotic cells are identified by their altered morphology, caspase activation, and the presence of damaged DNA.
本讲座全面讲解如何深入开展信号通路的研究,从大数据挖掘到锁定目标信号通路,进一步探讨疾病分子机制,并涉及到相关主流的实验技术,如修饰蛋白质组数据库使用、WB、ChIP、IHC、PTMScan蛋白质翻译后修饰高通量筛选技术等。
Our Insulin (C27C9) Rabbit mAb #3014 was produced with an antigenic peptide corresponding to residues within the insulin B chain of the human insulin protein (UniProt ID #P01308; https://www.uniprot.org/uniprot/P01308). Therefore, it is expected to recognize both proinsulin and mature insulin. While this antibody is not approved for use in western blotting, it should be able to detect the proinsulin protein if the gel is run carefully enough.We also offer an Insulin (L6B10) Mouse mAb #8138, which is approved for use in western blotting on rat samples. This antibody detects both proinsulin and insulin as well.
PTMScan Discovery uses immunoaffinity LC-MS with motif antibodies to identify and quantify PTMs on cellular proteins.
本讲座将概述细胞代谢相关的信号通路,并举例说明系列重要细胞代谢研究靶点,和精彩研究案例思路
Yes, you may freeze your cells and pool them for a later CUT&RUN experiment. However, we would suggest a light fixation using 0.1% formaldehyde for 2 min before freezing the cells, in case the protein-DNA association inside the nuclei changes during the freeze-thaw of the cells.
Marketing department will respond to signal pathway request. Link to tech support.
细胞焦亡与其他多种细胞程序性死亡形式之间存在相互联系和转化,共同构成细胞程序性死亡的复杂体系,维持细胞和机体的内稳态。
When developing the Malachite Green Phosphate Detection Kit #12776, we successfully used the Millipore Centrifuge filters AMICON ULTRA 0.5mL, 10,000 NMWL, product # UFC501096.
本场讲座将逐一介绍多种细胞程序性死亡(凋亡、自噬、程序性坏死、细胞焦亡)相关信号通路关键蛋白和研究策略,并就多种细胞程序性死亡形式之间的联系和转化展开讨论,为大家全面认识细胞程序性死亡和开展系统研究提供参考。
细胞程序性死亡(Programmed Cell Death, PCD),是指细胞接受某种信号或受到某些因素刺激后,为了维持内环境稳定而发生的一种主动性消亡过程。它
ChIP-seq is a powerful technique that combines ChIP and next-generation sequencing (NGS) to study DNA-protein interactions across the entire genome, but it is only as good as the quality of the antibody used in the ChIP experiment.
该视频将将逐一介绍参与自噬信号通路的关键分子,包括ULK1 、Beclin1、LC3、Atg5,ATG12,P62等
该视频将重点介绍与细胞骨架、细胞基质动态调控及EMT相关的主要调节分子和信号通路,并为各位听众提供在上述领域的主要评价指标作为参考。
Our testing on recombinant proteins indicates that the Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671 detects JNK1 (p46) and JNK2 (p54) well and JNK3 (p49) only weakly. Bands observed at 46kDa and 54kDa can be a mix of either JNK1 or JNK2, as each protein has multiple isoforms that run at these sizes.
该视频重点介绍肿瘤免疫微环境相关的主要参与细胞、调节分子和信号通路,并为各位听众提供在上述领域的主要评价指标和新的研究方法作为参考。
1.ChIP和ChIP seq的区别和应用范围; 2.ChIP和ChIP seq 实验的操作步骤和成功要素; 3.酶解和超声的优缺点及如何正确选择; 4.如何选择合适的用于ChIP和ChIP seq的抗体; 5.CST 标准 DNA文库建立方法及ChIP-Seq测序数据分析解读