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Pre-Clinical IHC Tools for Mouse Models
A scientific resource for the ANK protein domain containing information on structure, function, and binding partners.
Specific Aims set the stage for your grant proposal and are often considered the most important part, so make sure you're concisely conveying your goals.
The Rising Black Scientists Awards will be an annual prize. We will award many other worthy recipients in the future, but there’s something special about celebrating the first.
It remains difficult for scientists to predict the long-term health effects of COVID-19, particularly considering its diversity of effects during acute infection. The most severe impacts appear to manifest in tissues with high levels of vascularization (e.g., lungs, heart, kidney, liver), and it is evident that the virus elicits a significant inflammatory response in infected tissues
Our Anti-mouse IgG, HRP-linked Antibody #7076 shows approximately 25% reactivity to mouse IgM.
Webinar | Highly Multiplexed Single Cell Analysis of Tumor Heterogeneity through Time and Space by Mass Cytometry
Validated antibodies for high content screening from CST Cell Signaling Technology
The benefits of using micrococcal nuclease to digest chromatin compared to sonication.
A scientific resource for the BEACH protein domain containing information on structure, function, and phospholipid binding.
Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology
We perform our UV treatments as follows:
Complementary strategies provide vital information regarding antibody specificity or functionality and can be tailored to the nature of the downstream assay.
Chromatin IP (ChIP) Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
Our mCherry (E5D8F) Rabbit mAb #43590 was produced using a recombinant protein, of which we have yet to map the binding epitope. Although mCherry and RFP are homologous, they do not share 100% sequence identity. Therefore, there is a chance that #43590 will not detect RFP.
As a Development Scientist, I focused my attention on research developments in Alzheimer’s Disease (AD), the neurodegenerative disease that affects nearly 2.5 million patients in the U.S. alone.
We purchase our anti-IgM antibody from SouthernBiotech. It is Goat F(ab')2 Anti-Human IgM-UNLB (Cat. No.: 2022-01; https://www.southernbiotech.com/goat-f-ab-2-anti-human-igm-unlb-2022-01).
Scientific posters presented by CST scientists at various conferences and trade shows through the years.
No matter your research area, we’ve all needed to answer this deceptively easy question: how much of a particular protein is present in my samples?
The SARS-CoV-2 Spike Protein Serological IgG ELISA Kit #20154 kit is meant to be used to determine if the sample is positive or negative for SARS-CoV-2 and not for relative quantitation. The included positive control is meant to be used as an on/off signal to confirm that the assay is working and should have an OD signal greater than 1.5. This is not meant to be used for standard curve generation or quantitation.
Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells: easy to follow directions describing the step by step experimental procedure.
SignalScan bridges the gap between PCR-based detection of COVID-19 infection and uncovering the molecular signature of infection and host immune response.
Can a cellular identity crisis lead to cancer progression?
Our BACE (D10E5) Rabbit mAb #5606 was generated with an antigenic peptide that corresponds to residues surrounding His490 of the human BACE1 protein. The antigenic peptide shares little to no sequence identity with human BACE2. Therefore, this product is not expected to recognize BACE2.
We have only tested our Senescence β-Galactosidase Staining Kit #9860 with adherent cells. However, we commonly recommend immobilizing suspension cells to a glass slide by using a cytospin. Once the cells are mounted to the slide, you can follow our recommended protocol. Our customers have had success using this method.
The sequence of SUMO-4 is the same as SUMO-1, -2, and -3 at the c-terminus, so it will be a good substrate for the PTMScan Sumoylation Remnant Motif Kit #92465. However, SUMO-5 does not have the TGG sequence and would not be captured by the K-e-GG antibody after WaLP digest.
We recommend using a microwave to bring slides to a boil in 1 mM EDTA, pH 8.0 followed by 15 minutes at sub-boiling temperature. Typically the power level must be adjusted during the 15 minute period so as to prevent excessive buffer loss. Slides do not need to be cooled on the bench top, but can be rinsed in running dH2O.
Bay State Bike Week and the Mass Commute Bicycle Challenge is a great event to promote cycling and build community spirit. CST is looking forward to Bike Week next year where we hope to break the 2,100 mile mark!
Transfected Only sensitivity means the antibody is sensitive enough to detect your protein of interest when over-expressed, however is not sensitive enough to detect endogenously expressed protein.
We originally recommended milk as the western blot diluent for our Phospho-p70 S6 Kinase (Thr389) (D5U1O) Rabbit mAb #97596. However, after additional testing, we found that dilution in BSA showed stronger signal by western blot. Since that time, the diluent has been switched back to milk because we have been seeing a potentially non-specific band just above the p85 band in non-human samples. Milk appears to reduce the appearance of this band without decreasing target signal. We have decided reducing the background is more important than the slight increase in signal.