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A scientific resource for the PDZ protein domain containing information on structure, function, and domain binding.
Overview of Adhesion and Extracellular Matrix signaling networks, antibodies and related reagents, interactive pathway diagrams, and technical resources.
Clustered regularly interspaced short palindromic repeats, or CRISPRs, are repetitive sequences (direct repeats) native to the prokaryote genome.
A kinome diagram showing the CAMK group of protein kinases from Cell Signaling Technology.
A novel study from the laboratory of Daniel Figeys begins to unravel the complexity of the microbiome of pediatric patients with Crohn’s disease.
Quit living in the past! The future of cars is electric.
The most sensitive antibody will depend on whether you are trying to detect Cas9 protein from Streptococcus pyogenes, Staphylococcus aureus, or Campylobacter jejuni. The Cas9 (7A9-3A3) Mouse mAb #14697 and Cas9 (E7M1H) XP Rabbit mAb #19526 are the most sensitive choices by western immunoblot for the detection of Cas9 from Streptococcus pyogenes; whereas, the Cas9 (S. aureus) (E4G3U) Rabbit mAb #51610 is the most sensitive choice by western immunoblot for detection of Cas9 from Staphylococcus aureus. The Cas9 (C. jejuni) Antibody #50561 is currently our only antibody for western immunoblot for detection of Cas9 from Campylobacter jejuni.
Kinase-disease associations table showing kinases, kinase groups, disease types, and the molecular biology basis for disease.
Information and case study data for the PTMScan Direct Apoptosis/Autophagy Service offered by CST.
PTMScan Discovery in Translational Research (A Case Study)
A chronological listing of case studies and peer-reviewed publications utilizing PTMScan proteomic technology developed by Cell Signaling Technology.
Information and case study data for the PTMScan Direct Tyrosine Kinases Service from Cell Signaling Technology.
Information and case study data for the PTMScan Direct Cell Cycle and DNA Damage Service offered by CST.
Our Cas9 (7A9-3A3) Mouse mAb #14697 was produced with a recombinant protein specific to the amino terminus of Cas9 from Streptococcus pyogenes (UniProt ID #Q99ZW2; https://www.uniprot.org/uniprot/Q99ZW2). The precise epitope has not been mapped. However, this antibody has been shown to detect nuclease-deficient Cas9 (dCas9) protein and Cas9 nickase (niCas9) protein (please see the western blot image on the product datasheet; https://media.cellsignal.com/pdf/14697.pdf). dCas9 is a D10A/H840A mutant and niCas9 is a D10A mutant.
Case Study 4: Exceptional Performance of XP Monoclonal Antibodies from Cell Signaling Technology.
Case Study 3: Exceptional Reproducibility of XP Monoclonal Antibodies from Cell Signaling Technology.
An introduction to human protein kinases: protein kinases are key regulators of cell function.
Case Study 2: Exceptional Sensitivity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Case Study 1: Exceptional Specificity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Information and case study data for the PTMScan Direct Ser/Thr Kinases Service, which allows for the targeted screening of a defined set of protein modification sites.
Information and case study data for the PTMScan Direct PI3K/Akt Service offered by CST, which allows for targeted screening of 105 proteins within the Akt pathway.
Human protein kinases overview: Learn about the largest and most functionally diverse gene family, the different types and their functions. Click here.
Yes, you may freeze your cells and pool them for a later CUT&RUN experiment. However, we would suggest a light fixation using 0.1% formaldehyde for 2 min before freezing the cells, in case the protein-DNA association inside the nuclei changes during the freeze-thaw of the cells.
For antigenic targets where expression of the protein is very low or unknown, the use of recombinant proteins or exogenous expression in a surrogate cell line may be necessary for antibody validation.
Annual grant application guidelines and project description criteria for funding from CST.
Validation using recombinant proteins or exogenous expression in a surrogate cell line can be used for targets with low levels of protein expression.
Learn more about new lab startup discounts at Cell Signaling Technology.
Expert-reviewed interactive pathway providing a current overview of the molecular and cellular biology of Alzheimer’s Disease.
The specificity of our AMPKα Antibody #2532 has been confirmed as follows:1) siRNAPMID: 28008135 (https://pubmed.ncbi.nlm.nih.gov/28008135/) Figure 2A2) shRNAPMID: 28553939 (https://pubmed.ncbi.nlm.nih.gov/28553939/) Figure 1b3) CRISPR/Cas9 PMID: 30415923 (https://pubmed.ncbi.nlm.nih.gov/30415923/) Figure 3A
The average diameter of each Concanavalin A bead particle is ~1.0µm while the size range of a cell is 0.1-100 µm (the most commonly used cell lines like HeLa, HepG2 and HEK293 all have a cell size > 10µm). Therefore, in most cases, a cell is coated by multiple beads. This has also been confirmed by inspection under a microscope.
