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Resources results for "ser404"

  • Products (270)
  • Resources (45)

PTMScan® PI3K/Akt Signaling

Information and case study data for the PTMScan Direct PI3K/Akt Service offered by CST, which allows for targeted screening of 105 proteins within the Akt pathway.

Tau Protein and Neurofibrillary Tangles

Tau is a microtubule-associated protein found in neurons, and its function is to stabilize axonal microtubules. Hyperphosphorylated tau is a hallmark of several neurodegenerative diseases including Alzheimer’s disease.

Neurodegeneration: Tau Protein and Neurofibrillary Tangles

While the Tau protein is phosphorylated in normal, healthy brain, it becomes hyperphosphorylated in neurodegenerative diseases, like Alzheimer's.

PTMScan® Ser/Thr Kinases

Information and case study data for the PTMScan Direct Ser/Thr Kinases Service, which allows for the targeted screening of a defined set of protein modification sites.

Matched Antibody Pairs

Find validated CST® matched antibody pairs and streamline the development of your High-Throughput ELISA-Like Immunoassays for screening.

PTMScan® Direct Cell Cycle & DNA Damage

Information and case study data for the PTMScan Direct Cell Cycle and DNA Damage Service offered by CST.

PTMScan® Direct Apoptosis & Autophagy

Information and case study data for the PTMScan Direct Apoptosis/Autophagy Service offered by CST.

PI3K / Akt Substrates Table

The PI3K / Akt Substrates Table provides a comprehensive list of demonstrated downstream targets of Akt phosphorylation.

Resources

Phosphorylation of serines 465 and 467 indicates Smad2 activation and nuclear localization; whereas, phosphorylation of serines 245, 250, and 255 is inhibitory and leads to retention of Smad2 in the cytoplasm.

Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108, Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb #8828, and Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit mAb #18338 recognize phosphosites at the carboxy-terminus of Smad2 that are phosphorylated in response to TGF-beta treatment. Phospho-Smad2 (Ser245/250/255) Antibody #3104 instead recognizes a cluster of phosphosites in the Smad2 linker region that are phosphorylated by MAP kinase.  
 

Resources

Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108, Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb #8828, Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit mAb #18338, and Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520 are performance tested on extracts prepared from serum-starved cells that have been treated with 10ng/ml TGF-beta3 for 30 minutes.  It is possible that longer treatments (e.g., 24 hours) can lead to diminished signals for phospho-Smad2 and phospho-Smad3 because of Smad7 induction. 

​​Failure to include the appropriate serine/threonine phosphatase inhibitors can result in a diminished signal and/or inconsistent results between experiments. We always include sodium pyrophosphate (2.5mM final) and beta-glycerophosphate (1.0mM final) as serine/threonine phosphatase inhibitors in the lysis buffer for detection of the phospho-Smads. Our Phosphatase Inhibit…

Cytokines and Inflammation

Products and Related Resources for Cytokines and Inflammation SARS-CoV-2 Research

Fibrosis

Products and Related Resources for Fibrosis SARS-CoV-2 Research

Safeguard the Quality of Your ELISA Data: Ensuring ELISA Lot-to-Lot Reproducibility

Testing ELISA kit lots for percent coefficient of variation (CV), signal/blank ratios, and optical density helps ensure scientific reproducibility.

Ubiquitin Ligase Table

The Ubiquitin Ligase Table provides a comprehensive list of E3 ubiquitin ligases along with their substrates and corresponding PubMed reference(s).

Viral Entry, Replication, Transcription, and Translation

Products and Related Resources for Viral Entry, Replication, and Transcription SARS-CoV-2 Research

Publications By Our Scientists

A comprehensive list of peer-reviewed publications from Cell Signaling Technology.

Resources

The amino acid region surrounding the Ser463/465 phospho sites is well conserved in Smad1, 5, and 9. An alignment using the human sequences found in UniProt shows only one residue difference in this region. While we have performed testing to demonstrate that our Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb #13820 binds phospho-Smad9, we have not performed similar testing with our Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516. Based on sequence conservation we would predict that #9516 may cross-react with phospho-Smad9, as we indicate in the Specificity/Sensitivity statement on our datasheet and product webpage. However, until we perform the necessary experiments we cannot guarantee phospho-Smad9 recognition using #9516.

Resources

We believe the upper band observed when using the Rad18 (D2B8) XP® Rabbit mAb #9040, Phospho-Rad18 (Ser403) (D2T6W) Rabbit mAb #14978, or Phospho-Rad18 (Ser403) Antibody #8393 is likely mono-ubiquitinated Rad18 (see Inagaki, A et al. (2011) PLoS One. 6(8), e23155 PMID: 21858012; https://www.ncbi.nlm.nih.gov/pubmed/21858012).  This target may also have other modifications, such as phosphorylation, due to two bands being seen with the phospho-antibodies.

Resources

Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 and Phospho-Akt (Ser473) (D9W9U) Mouse mAb #12694 detect over-expressed PKC alpha and beta. Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 also detects endogenous levels of PKC in Jurkat + CalA extract, but not in other endogenous models tested. Our Phospho-Akt (Ser473) (587F11) Mouse mAb #4051 detects over-expressed PKC alpha and beta but does not detect endogenous PKC in the models tested. Our Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058 does not detect over-expressed or endogenous PKC alpha and beta.

Resources

  • Our Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 and Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb #13820 are performance tested on extracts prepared from serum-starved cells treated with 50ng/ml BMP-2 for 30 minutes.
 

Parkinson’s Disease Resource Center

Resources

It is possible to see a doublet with our Phospho-c-Jun (Ser73) (D47G9) XP Rabbit mAb #3270 in some extracts. This is because #3230 can recognize JunD phosphorylated at Ser100. Phospho-c-Jun (Ser73) is the higher band at approximately 48 kDa, whereas phospho-JunD (Ser100) is the lower band at approximately 42 kDa.

Resources

The Ser485 site detected by our Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb #2537 has been identified as an inhibitory phosphosite of APMKα1 and will be phosphorylated to a lesser degree when AMPK is activated in response to nutrient-deprived conditions (e.g., serum or glucose starvation). Therefore, you should see a decrease in signal intensity with #2537 in serum-starved samples, such as the positive extract included in our AMPK Control Cell Extracts #9158.

Virus Detection and Innate Immune Activation

Products and Related Resources for Virus Detection and Innate Immune Activation SARS-CoV-2 Research

Stem Cell and Lineage Markers

The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.

Overview of Cell Proliferation

Cell viability assays measure the population of live, viable cells within a sample. Typically, viability assays measure markers of cell health, including cellular metabolism, ATP levels, and cell proliferation.

PI3K / Akt Signaling

Discover the PI3K Akt pathway and its crucial role in cell growth and survival. Click here to learn more & gain insights into this important signaling cascade

ChIP Validated Antibodies and Primer Pairs

SimpleChIP Kits and Antibodies validated in-house by our antibody development scientists correlated to positive and negative control primers.

Akt Antibody Comparison Tables

Compare Cell Signaling Technology's Akt antibodies against one another to determine optimal applications