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Resources results for "5-HT(1A)"

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Cell Death and Disease

Hallmarks of Antibody Validation: Ranged Strategy

Hallmarks of Antibody Validation: Ranged Strategy. Binary models are not always readily available and can be time-consuming to produce for validating an antibody.

Hallmarks of Validation - Ranged Strategy

A ranged validation strategy includes both endogenous and heterologous models that express high, moderate, and low levels of the target of interest.

Overview of Cell Death

Apoptosis is a type of programmed cell death that occurs normally throughout the lifespan and can also occur in response to harmful stimuli. Apoptotic cells are identified by their altered morphology, caspase activation, and the presence of damaged DNA.

Overview of the Immune System

The immune system is composed of tissues, cells, and molecules whose primary function is to detect, respond to, and eliminate pathogens and transformed cells.

Regulation of Actin Dynamics

Expert-reviewed interactive pathway providing a current overview of Regulation of Actin Dynamics.

Cytoskeletal Regulation and Vesicle Trafficking

Expert-reviewed interactive signaling pathways providing current overviews of the regulation of actin and microtubule dynamics, as well as links to products from CST.

Successful Immunofluorescence: Antibody Dilution & Incubation Conditions

Changing the primary antibody dilution and incubation conditions affects fluorescence intensity and signal-to-noise ratio in IF experiments.

10 Must-have Markers for Senescence Research

Interested in studying senescence? Understanding cell cycle arrest is critical to many fields of research, including development, aging, and cancer.

Exceptional Performance of XP® Monoclonal Antibodies

Case Study 4: Exceptional Performance of XP Monoclonal Antibodies from Cell Signaling Technology.

Increase Your Experimental Success with CUT&RUN-Validated Antibodies

Do ChIP-validated antibodies work in CUT&RUN assays? Not necessarily. We found only 50-60% of ChIP- or ChIP-seq antibodies were compatible with CUT&RUN.

Successful Immunofluorescence: Experimental Controls

Knockout cell lines and modulating the target's phosphorylation state are experimental controls that can be used to confirm specificity of your cell type.

Understanding the Role of RNA Regulation and Epitranscriptomics in Disease

Learn how RNA-binding proteins influence RNA regulation and epitranscriptomics and contribute to the progression of a variety of diseases.

Emerging Therapeutic Approaches for Prostate Cancer

Roughly 1 in 8 men will be diagnosed with prostate cancer. So why do so many men lose their battle? Emerging therapeutic options may turn this around.

Immunotherapy: Empowering Nature’s Anti-tumor Artillery - Part II

Advancements in immunotherapeutics against cancer include receptor agonists, adoptive cell transfer, anti-cancer vaccines, and combination therapies.

Hallmarks of Antibody Validation: Binary Strategy

A binary approach is one of the best ways to evaluate antibody specificity. By testing an antibody in biologically relevant positive and negative expression systems.

Necroptosis and Pyroptosis Add to Our Understanding of Regulated Cell Death

These markers can be used to study necroptosis and pyroptosis, forms of regulated necrotic cell death that share morphological features with necrosis.

Hallmarks of Antibody Validation - Binary Strategy

A binary approach is one of the best ways to evaluate antibody specificity.

Solutions for Oncology Therapeutics Discovery

Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.

Protein and Pathway Abbreviations

Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.

Neurodegeneration Drug Development

Streamline your neurodegeneration therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.

Publications By Our Scientists

A comprehensive list of peer-reviewed publications from Cell Signaling Technology.

Resources

Bmi1 undergoes numerous post-translational modifications and can be heavily phosphorylated, as described on the PhosphoSite.org protein page (https://www.phosphosite.org/proteinAction.action?id=1286622&showAllSites=true). Therefore, we believe the upper band represents phosphorylated Bmi1.

Multiple papers have also described Bmi1 migrating as a doublet by western blot, as shown in the examples below.

Resources

MAFA is a phosphoprotein. The multiple phosphorylation sites on this protein cause a mobility shift, resulting in the protein migrating slower by gel electrophoresis. This has been confirmed by treatment with CIAP, which has been shown to result in a shift in MAFA’s mobility from approximately 48 kDa to a more rapidly migrating form of approximately 40 kDa (see PMID: 17682063 Figure 1A; https://pubmed.ncbi.nlm.nih.gov/17682063/).



 

Immunohistochemistry (IHC) Troubleshooting Guide & the Importance of Using Controls

Eliminate non-specific binding and high background with the immunohistochemitry (IHC) troubleshooting guide and positive/negative controls

Validated CST® Antibodies Work on the Cell DIVE Multiplexed Imaging Solution

PTMScan® Motif Antibody Kits

PTMScan Motif Antibody Kits from Cell Signaling Technology

What is Senescence?

Cellular senescence is defined by permanent cell cycle arrest. Senescent cells accumulate with age and contribute to normal aging and age-related disorders

Regulation of Apoptosis

Study the intricate machinery of the apoptosis signaling pathway. Click here to learn more and gain insights into programmed cell death mechanisms.

Resources

CIP/Lambda Phosphatase Treatment of Cell Lysates:

1) Wash cells 2X with ice-cold PBS.

2) Add cold 1X cell lysis buffer that does not include phosphatase inhibitors [1-2 E7 cells per ml or 0.4ml per 10 cm plate (80-90% confluent)]. Our cell lysis buffer is prepared as follows:

20 mM Tris-HCl (pH 7.5)
150 mM NaCl
1 mM Na2EDTA
1 mM EGTA
1% Triton
1 ug/ml leupeptin
1mM PMSF

3) Add 20ul Quick CIP (NEB #M0525; https://www.neb.com/products/m0525-quick-cip#Product%20Information) per 400ul of cell extract.

4) Incubate with Quick CIP at 37C for 1 hour.

5) Add 20ul lambda protein phosphatase (CST