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Study the intricate Notch signaling pathway and its impact on cell fate determination and development. Click here to learn more.
Harnessing the power of the immune system's T cells to attack and treat cancer is a promising research area that may lead to highly effective therapies.
Richard discusses a recent paper that investigates the potential mechanistic link between the AD risk gene APOE4 and altered metabolism. This makes an interesting potential connection from diabetes to Alzheimer's.
p16 is an important cervical cancer biomarker but studies have shown variable expression levels, suggesting an IHC-validated p16 INK4A antibody is needed.
CST Technical Support Articles
Our Anti-biotin, HRP-linked Antibody #7075 is intended for use with our Biotinylated Protein Ladder Detection Pack #7727. However, it is possible for this antibody to also detect endogenously biotinylated proteins in cell lysates if present in sufficient quantities. If this type of interaction is suspected in a given experiment, it is recommended that the Biotinylated Protein Ladder be probed separately from the cell lysate lanes to avoid detection of endogenously biotinylated proteins in the experimental samples.
Expert-reviewed interactive pathway providing a current overview of Nuclear Receptor Signaling.
Explore the NFkB pathway and its impact on inflammation and immune responses. Click here to learn more about this signaling pathway.
The typical DNA yields from ChIP experiments range from 0.2-2 ng/µl when targeting transcription factors or cofactors and from 2-20 ng/µl when targeting histone modifications. If the concentration is within this range, then you should be able to continue with ChIP-seq. Our DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795 can generate a library with 0.5ng ChIP-DNA.
Unfortunately, we are unable to provide the Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product. This product is meant for use, in tandem, with our pAG-MNase Enzyme #57813, which is a proprietary product.
Upon arrival of the kit to your facility, the components should be broken down and stored at their recommended temperatures. We have conducted stability tests for a few components of the kit and found a decrease in signal when using Digitonin Solution kept at 4C. We have never tested Spermidine nor 4X Stop Buffer for 4C stability therefore we do not have any definitive data, however we believe these components could become inactive if kept above -20C for prolonged periods of time.
Discover the mTOR pathway and its role in cellular growth and metabolism. Click here to explore the intricacies of this vital signaling pathway.
Histones have numerous post-translational modifications (PTMs), which can cause the protein to run at a different rate during electrophoresis. The upper band that migrates at ~25-27 kDa is likely phosphorylated histone H2A.X which also contains additional PTMs, such as ubiquitination. We believe this to be the case as the signal of the phosphorylated histone H2A.X band at 15 kDa and the upper band at 25-27kDa both increase in signal strength upon UV treatment. In addition, the 25-27kDa upper band can be seen with our total histone H2A antibodies (#7631, #2595, #50722), in both untreated and treated samples, indicating that it is specific to histone H2A.
Explore the G protein coupled receptor signaling pathway and its diverse roles in cellular communication. Read more here.
Study the IL-6 pathway and its impact on inflammation and immune regulation. Delve into the intricacies of this signaling cascade. Click here.
Expert-reviewed interactive pathway providing a current overview of ErbB/HER Signaling.
Explore the ERK pathway and its role in cell proliferation and survival. Click here to read more about this critical signaling pathway.
Researchers combined their expertise to successfully pioneer a new path towards identifying the ubiquitous protein modification O-GlcNAcylation.
Theoretically, our 5-Methylcytosine (5-mC) (D3S2Z) Rabbit mAb #28692 should detect the RNA m5C modification but we have not specifically tested this in-house.
Co-staining with multiple antibodies is a low-content form of multiplex analysis that be achieved using different host species or fluorophore conjugates.
GPNMB is an attractive therapeutic target for antibody-based therapeutics, and is currently being evaluated for its potential in treating triple-negative breast cancer and other metastatic cancers. Let's explore the strategy behind one type of immunotherapeutics, antibody-drug conjugates (ADCs).
A research timeline can demonstrate that you've considered project feasibility and future directions show that your plans span beyond the current proposal.
An orthogonal antibody validation strategy is critical to verify existing validation data and identify any effects or artifacts that are directly related.
We have performed side-by-side testing of the Caspase-3 Activity Assay Kit #5723, with and without PMSF, and find that the assay performs better without the addition of PMSF. The PathScan Sandwich ELISA Lysis Buffer (1X) #7018 has been optimized for use with the Caspase-3 Activity Assay Kit #5723 without the addition of supplemental inhibitors, such as PMSF.
Discover the insulin signaling pathway and its role in glucose metabolism & diabetes. Learn more about insulin action here.
The concentration of our Glycine Solution (10X) #7005 is 1.25M and the pH is 6.5.
You’re at an impasse. How should you go about narrowing the scope of your discovery efforts? It sounds like you need an antibody sampler kit.
Hypoxia causes cellular stress and alters normal metabolic activity. These pathways allow cells to survive and recover during bouts of low oxygen.
We have not tested the reactivity of our myosin light chain 2 antibodies against myosin 12a/b (UniProt: P19105 and O14950). However, based on homology, there is a good chance for cross-reactivity. The length of MLC12a/b is 171 amino acids and 172 amino acids respectively and both are predicted to run at ~20 kDa.The peptide immunogens used to produce our Myosin Light Chain 2 antibodies have the following sequence homology with Myosin 12a and 12b: