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Information and case study data for the PTMScan Direct Apoptosis/Autophagy Service offered by CST.
The specificity of our Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 has been confirmed as follows:1) see the western image on the product webpage at https://www.cellsignal.com/products/primary-antibodies/phospho-ampka-thr172-40h9-rabbit-mab/2535. The specificity of #2535 has been confirmed on a binary model (i.e., on extracts from C2C12 cells, untreated or oligomycin-treated (0.5 µM). 2) shRNAPMID: 28553939 (https://pubmed.ncbi.nlm.nih.gov/28553939/) Figure 1b3) CRISPR/Cas9 PMID: 30415923 (https://pubmed.ncbi.nlm.nih.gov/30415923/) Figure 3A
The Kinase Library can determine the kinases likely to phosphorylate any given motif. Learn about the research enabling this phosphoproteomics revolution.
Overview of methodology and client deliverables for the PTMScan Direct Proteomic Service offered by Cell Signaling Technology.
How are antibodies used in research? What is antibody affinity, and how is it determined? Part 1 Antibody Essentials answers these questions and more.
Information on KinomeView western blotting service offered by Cell Signaling Technology that uses Phospho-Motif Antibodies to provide a kinome-wide view of cellular phosphorylation.
Discover the autophagy pathway and its crucial role in cellular homeostasis. Learn more and uncover the mechanisms of cellular self-degradation here.
Partnerships & Licensing information
The ubiquitin signal can be increased by using a semi-dry-transfer and autoclaving the membrane for 30 minutes on a wet cycle or boiling the membrane for 30 minutes after transfer.
CST® Proteomics Analytical Services help you identify and validate drug targets, discover biomarkers, determine on/off-target effects, and mechanisms of action.
Cell Signaling Technology announces the launch of SignalStar™ Multiplex IHC, a flexible spatial biology solution for up to 8-plex amplification in two days.
Troubleshooting
A video tutorial illustrating the methodology behind PTMScan Technology and an example of its use in a recent phosphoproteomic study authored by scientists from CST.
Download white paper guides to key CST applications: western blotting and iPSC generation.
What Assay should you be for Halloween? Western Blot, IHC, IF, Flow Cytometry, ChIP, or ELISA...
The ChIP Sonication Nuclear Lysis Buffer #28778 included in the SimpleChIP® Sonication Cell and Nuclear Lysis Buffers #81804 contains 0.1% SDS.
CST can identify and quantify thousands of post-translational modification sites specific to your sample—phosphorylation, ubiquitination, acetylation, and more.
CUT&Tag Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
How much antibody should I use for chromatin immunoprecipitation? Watch this Tech Tips video to learn why more antibody isn't always better.
Cell Signaling Technology (CST) Enters Agreement with Roche for CDx Development
Tailor orders to have product when you need it. Make single-lot reservations and order bulk quantities of CST® antibodies, buffers, and ELISA kits.
"PI3K/Akt signaling is close to my heart because certain diseases that have cropped up in my family are linked to specific proteins in this pathway."
CST antibodies have been validated for Simple Western, the only platform on the market that provides a fully automated western blotting workflow.
PTMScan Motif Antibodies and Kits, for customers who purchase a license and would like to perform PTMScan Proteomics in-house.
The immune system is composed of tissues, cells, and molecules whose primary function is to detect, respond to, and eliminate pathogens and transformed cells.
When using antibodies for CUT&RUN against histone modifications, we typically see fragment sizes as small as 150 bp (size of a mono-nucleosome) and quite often see a nucleosomal ladder of DNA fragment sizes (i.e. 150, 300, 450, 600 bp). When using antibodies against transcription factors, we see a range of DNA fragment sizes, with the majority being greater than 35 bp.
If your DNA fragments are larger than 500 base pairs, we recommend sonicating to form 200-500 base pair fragments that will more readily denature. Sonication should be performed prior to starting the MeDIP or dot blot protocol. DNA fragment size can be analyzed by gel electrophoresis on a 1% agarose gel with a 100 bp DNA marker.
Cell Signaling Technology IHC technical support contact information and outline of requested information that our technical support specialists will need to assist you.
The β-Actin (8H10D10) Mouse mAb #3700 has kappa light chains.
Lieping Chen, Yale University Cancer Center, Awarded Cell Signaling Technology Innovation and Translational Research Award for Outstanding Scientists of Chinese Heritage